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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Semicontinuous Bioreactor Production of Recombinant Butyrylcholinesterase in Transgenic Rice Cell Suspension Cultures
|
|---|---|
| Published in |
Frontiers in Plant Science, March 2016
|
| DOI | 10.3389/fpls.2016.00412 |
| Pubmed ID | |
| Authors |
Jasmine M. Corbin, Bryce I. Hashimoto, Kalimuthu Karuppanan, Zachary R. Kyser, Liying Wu, Brian A. Roberts, Amy R. Noe, Raymond L. Rodriguez, Karen A. McDonald, Somen Nandi |
| Abstract |
An active and tetrameric form of recombinant butyrylcholinesterase (BChE), a large and complex human enzyme, was produced via semicontinuous operation in a transgenic rice cell suspension culture. After transformation of rice callus and screening of transformants, the cultures were scaled up from culture flask to a lab scale bioreactor. The bioreactor was operated through two phases each of growth and expression. The cells were able to produce BChE during both expression phases, with a maximum yield of 1.6 mg BChE/L of culture during the second expression phase. Cells successfully regrew during a 5-day growth phase. A combination of activity assays and Western blot analysis indicated production of an active and fully assembled tetramer of BChE. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 2 | 67% |
| Italy | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 2% |
| Unknown | 59 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 25% |
| Student > Bachelor | 9 | 15% |
| Student > Master | 8 | 13% |
| Researcher | 4 | 7% |
| Lecturer | 2 | 3% |
| Other | 7 | 12% |
| Unknown | 15 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 22% |
| Agricultural and Biological Sciences | 10 | 17% |
| Chemical Engineering | 6 | 10% |
| Engineering | 4 | 7% |
| Chemistry | 4 | 7% |
| Other | 3 | 5% |
| Unknown | 20 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 February 2017.
All research outputs
#13,463,735
of 22,858,915 outputs
Outputs from Frontiers in Plant Science
#6,683
of 20,216 outputs
Outputs of similar age
#146,867
of 301,001 outputs
Outputs of similar age from Frontiers in Plant Science
#150
of 504 outputs
Altmetric has tracked 22,858,915 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,216 research outputs from this source. They receive a mean Attention Score of 4.0. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 301,001 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 504 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.