Title |
Rapid Identification of Officinal Akebiae Caulis and Its Toxic Adulterant Aristolochiae Manshuriensis Caulis (Aristolochia manshuriensis) by Loop-Mediated Isothermal Amplification
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Published in |
Frontiers in Plant Science, June 2016
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DOI | 10.3389/fpls.2016.00887 |
Pubmed ID | |
Authors |
Lan Wu, Bo Wang, Mingming Zhao, Wei Liu, Peng Zhang, Yuhua Shi, Chao Xiong, Ping Wang, Wei Sun, Shilin Chen |
Abstract |
Mu-tong (Akebiae Caulis) is a traditional Chinese medicine commonly used as a diuretic and antiphlogistic. A common adulterant of Mu-tong is Guan-mu-tong (Aristolochiae Manshuriensis Caulis), which is derived from the stem of Aristolochia manshuriensis Komarov, and contains carcinogenic aristolochic acids. We used an alternative technique, loop-mediated isothermal amplification (LAMP), to differentiate Mu-tong from Guan-mu-tong because LAMP is quick, highly sensitive, and specific. We designed a set of four common primers (G-F3, G-B3, G-FIP, and G-BIP) and a loop primer (G-LB) for LAMP based on the internal transcribed spacer 2 sequence of Ar. manshuriensis. We successfully amplified the LAMP assays and visual detection occurred within 60 min at isothermal conditions of 65°C. The LAMP reaction exhibited a tenfold increase in detection (4.22 pg/μl DNA) over conventional polymerase chain reaction demonstrating that LAMP is a useful technique to detect Guan-mu-tong. We conclude that the LAMP technique is a potentially valuable safety control method for simple and efficient discrimination of Mu-tong from its adulterant Guan-mu-tong. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor > Associate Professor | 2 | 13% |
Researcher | 2 | 13% |
Unspecified | 1 | 7% |
Student > Ph. D. Student | 1 | 7% |
Other | 1 | 7% |
Other | 2 | 13% |
Unknown | 6 | 40% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 5 | 33% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 13% |
Veterinary Science and Veterinary Medicine | 1 | 7% |
Unspecified | 1 | 7% |
Earth and Planetary Sciences | 1 | 7% |
Other | 1 | 7% |
Unknown | 4 | 27% |