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Benzophenone Synthase and Chalcone Synthase Accumulate in the Mesophyll of Hypericum perforatum Leaves at Different Developmental Stages

Overview of attention for article published in Frontiers in Plant Science, June 2016
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Title
Benzophenone Synthase and Chalcone Synthase Accumulate in the Mesophyll of Hypericum perforatum Leaves at Different Developmental Stages
Published in
Frontiers in Plant Science, June 2016
DOI 10.3389/fpls.2016.00921
Pubmed ID
Authors

Asma K. Belkheir, Mariam Gaid, Benye Liu, Robert Hänsch, Ludger Beerhues

Abstract

The active medicinal constituents in Hypericum perforatum, used to treat depression and skin irritation, include flavonoids and xanthones. The carbon skeletons of these compounds are formed by chalcone synthase (CHS) and benzophenone synthase (BPS), respectively. Polyclonal antisera were raised against the polyketide synthases from Hypericum androsaemum and their IgG fractions were isolated. Immunoblotting and immunotitration were used to test the IgGs for crossreactivity and monospecificity in H. perforatum leaf protein extract. Immunofluorescence localization revealed that both CHS and BPS are located in the mesophyll. The maximum fluorescence levels were observed in approx. 0.5 and 1 cm long leaves, respectively. The fluorescence intensity observed for CHS significantly exceeded that for BPS. Using histochemical staining, flavonoids were detected in the mesophyll, indicating that the sites of biosynthesis and accumulation coincide. Our results help understand the biosynthesis and underlying regulation of active H. perforatum constituents.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Slovakia 1 2%
Unknown 50 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 24%
Student > Bachelor 5 10%
Student > Master 4 8%
Researcher 3 6%
Other 3 6%
Other 6 12%
Unknown 18 35%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 27%
Biochemistry, Genetics and Molecular Biology 5 10%
Pharmacology, Toxicology and Pharmaceutical Science 3 6%
Medicine and Dentistry 3 6%
Engineering 3 6%
Other 3 6%
Unknown 20 39%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 June 2016.
All research outputs
#20,334,427
of 22,879,161 outputs
Outputs from Frontiers in Plant Science
#16,165
of 20,270 outputs
Outputs of similar age
#304,955
of 352,012 outputs
Outputs of similar age from Frontiers in Plant Science
#405
of 526 outputs
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So far Altmetric has tracked 20,270 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 526 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.