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Ascorbate-Deficient vtc2 Mutants in Arabidopsis Do Not Exhibit Decreased Growth

Overview of attention for article published in Frontiers in Plant Science, July 2016
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Title
Ascorbate-Deficient vtc2 Mutants in Arabidopsis Do Not Exhibit Decreased Growth
Published in
Frontiers in Plant Science, July 2016
DOI 10.3389/fpls.2016.01025
Pubmed ID
Authors

Benson Lim, Nicholas Smirnoff, Christopher S. Cobbett, John F. Golz

Abstract

In higher plants the L-galactose pathway represents the major route for ascorbate biosynthesis. The first committed step of this pathway is catalyzed by the enzyme GDP-L-galactose phosphorylase and is encoded by two paralogs in Arabidopsis - VITAMIN C2 (VTC2) and VTC5. The first mutant of this enzyme, vtc2-1, isolated via an EMS mutagenesis screen, has approximately 20-30% of wildtype ascorbate levels and has been reported to have decreased growth under standard laboratory conditions. Here, we show that a T-DNA insertion into the VTC2 causes a similar reduction in ascorbate levels, but does not greatly affect plant growth. Subsequent segregation analysis revealed the growth defects of vtc2-1 mutants segregate independently of the vtc2-1 mutation. These observations suggest that it is the presence of an independent cryptic mutation that affects growth of vtc2-1 mutants, and not the 70-80% decrease in ascorbate levels that has been assumed in past studies.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 64 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 25%
Researcher 10 16%
Student > Master 10 16%
Student > Doctoral Student 6 9%
Professor 3 5%
Other 5 8%
Unknown 14 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 52%
Biochemistry, Genetics and Molecular Biology 10 16%
Business, Management and Accounting 1 2%
Unspecified 1 2%
Nursing and Health Professions 1 2%
Other 1 2%
Unknown 17 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 August 2023.
All research outputs
#15,936,348
of 24,255,619 outputs
Outputs from Frontiers in Plant Science
#10,364
of 22,737 outputs
Outputs of similar age
#224,170
of 361,396 outputs
Outputs of similar age from Frontiers in Plant Science
#204
of 518 outputs
Altmetric has tracked 24,255,619 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 22,737 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 361,396 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 518 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.