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Attention Score in Context
| Title |
Induction of Silencing in Plants by High-Pressure Spraying of In vitro-Synthesized Small RNAs
|
|---|---|
| Published in |
Frontiers in Plant Science, August 2016
|
| DOI | 10.3389/fpls.2016.01327 |
| Pubmed ID | |
| Authors |
Athanasios Dalakouras, Michèle Wassenegger, John N. McMillan, Vinitha Cardoza, Ira Maegele, Elena Dadami, Miriam Runne, Gabi Krczal, Michael Wassenegger |
| Abstract |
In this report, we describe a method for the delivery of small interfering RNAs (siRNAs) into plant cells. In vitro synthesized siRNAs that were designed to target the coding region of a GREEN FLUORESCENT PROTEIN (GFP) transgene were applied by various methods onto GFP-expressing transgenic Nicotiana benthamiana plants to trigger RNA silencing. In contrast to mere siRNA applications, including spraying, syringe injection, and infiltration of siRNAs that all failed to induce RNA silencing, high pressure spraying of siRNAs resulted in efficient local and systemic silencing of the GFP transgene, with comparable efficiency as was achieved with biolistic siRNA introduction. High-pressure spraying of siRNAs with sizes of 21, 22, and 24 nucleotides (nt) led to local GFP silencing. Small RNA deep sequencing revealed that no shearing of siRNAs was detectable by high-pressure spraying. Systemic silencing was basically detected upon spraying of 22 nt siRNAs. Local and systemic silencing developed faster and more extensively upon targeting the apical meristem than spraying of mature leaves. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 158 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 158 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 40 | 25% |
| Researcher | 30 | 19% |
| Student > Bachelor | 15 | 9% |
| Student > Master | 9 | 6% |
| Other | 6 | 4% |
| Other | 15 | 9% |
| Unknown | 43 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 63 | 40% |
| Biochemistry, Genetics and Molecular Biology | 39 | 25% |
| Environmental Science | 3 | 2% |
| Arts and Humanities | 2 | 1% |
| Chemical Engineering | 1 | <1% |
| Other | 4 | 3% |
| Unknown | 46 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 February 2017.
All research outputs
#14,861,191
of 22,888,307 outputs
Outputs from Frontiers in Plant Science
#9,317
of 20,287 outputs
Outputs of similar age
#205,085
of 336,879 outputs
Outputs of similar age from Frontiers in Plant Science
#167
of 429 outputs
Altmetric has tracked 22,888,307 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,287 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 336,879 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 429 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.