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Isolation and Functional Characterization of a Lycopene β-cyclase Gene Promoter from Citrus

Overview of attention for article published in Frontiers in Plant Science, September 2016
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Title
Isolation and Functional Characterization of a Lycopene β-cyclase Gene Promoter from Citrus
Published in
Frontiers in Plant Science, September 2016
DOI 10.3389/fpls.2016.01367
Pubmed ID
Authors

Suwen Lu, Yin Zhang, Xiongjie Zheng, Kaijie Zhu, Qiang Xu, Xiuxin Deng

Abstract

Lycopene β-cyclases are key enzymes located at the branch point of the carotenoid biosynthesis pathway. However, the transcriptional regulatory mechanisms of LCYb1 in citrus with abundant carotenoid accumulation are still unclear. To understand the molecular basis of CsLCYb1 expression, we isolated and functionally characterized the 5' upstream sequences of CsLCYb1 from citrus. The full-length CsLCYb1 promoter and a series of its 5' deletions were fused to the β-glucuronidase (GUS) reporter gene and transferred into different plants (tomato, Arabidopsis and citrus callus) to test the promoter activities. The results of all transgenic species showed that the 1584 bp upstream region from the translational start site displayed maximal promoter activity, and the minimal promoter containing 746 bp upstream sequences was sufficient for strong basal promoter activity. Furthermore, the CsLCYb1 promoter activity was developmentally and tissue-specially regulated in transgenic Arabidopsis, and it was affected by multiple hormones and environmental cues in transgenic citrus callus under various treatments. Finer deletion analysis identified an enhancer element existing as a tandem repeat in the promoter region between -574 to -513 bp and conferring strong promoter activity. The copy numbers of the enhancer element differed among various citrus species, leading to the development of a derived simple sequence repeat marker to distinguish different species. In conclusion, this study elucidates the expression characteristics of the LCYb1 promoter from citrus and further identifies a novel enhancer element required for the promoter activity. The characterized promoter fragment would be an ideal candidate for genetic engineering and seeking of upstream trans-acting elements.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 19%
Student > Bachelor 4 13%
Student > Master 4 13%
Student > Ph. D. Student 3 10%
Lecturer 1 3%
Other 3 10%
Unknown 10 32%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 39%
Biochemistry, Genetics and Molecular Biology 4 13%
Chemical Engineering 1 3%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Psychology 1 3%
Other 1 3%
Unknown 11 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 September 2016.
All research outputs
#15,333,342
of 22,888,307 outputs
Outputs from Frontiers in Plant Science
#10,672
of 20,287 outputs
Outputs of similar age
#202,700
of 322,146 outputs
Outputs of similar age from Frontiers in Plant Science
#198
of 433 outputs
Altmetric has tracked 22,888,307 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,287 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 322,146 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 433 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.