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De novo Transcriptome Analysis Revealed Genes Involved in Flavonoid and Vitamin C Biosynthesis in Phyllanthus emblica (L.)

Overview of attention for article published in Frontiers in Plant Science, October 2016
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Title
De novo Transcriptome Analysis Revealed Genes Involved in Flavonoid and Vitamin C Biosynthesis in Phyllanthus emblica (L.)
Published in
Frontiers in Plant Science, October 2016
DOI 10.3389/fpls.2016.01610
Pubmed ID
Authors

Avneesh Kumar, Sunil Kumar, Savita Bains, Vanya Vaidya, Baljinder Singh, Ravneet Kaur, Jagdeep Kaur, Kashmir Singh

Abstract

Phyllanthus emblica is an affluent source of various therapeutic components. A few of them like vitamin C and flavonoids are predominant bioactive compounds that are being used in immense pharmacological applications. In-spite of numerous applications, the genomic information of this plant was limited to a few expressed sequence tags (ESTs) in DNA databases. Herein, we developed in-depth transcriptome information of P. emblica using Illumina Hiseq 2000 platform and characterized. A total of 31,285,965 high-quality reads were assembled into 91,288 contigs with the N50 value 358. Out of them, 47,267 contigs were functionally annotated using BLASTX search against NCBI-non-redundant (NR) protein database. Further, 31,366 contigs showed similarity with various gene ontology (GO) terms, and 1299 were related to different enzymes and biosynthetic pathways. We identified the transcripts related to each gene involved in flavonoid and vitamin C biosynthesis. Several cytochrome P450s (CYPs) and glucosyltransferases (GTs) genes involved in flavonoid biosynthesis and various other metabolic pathways were also documented. Further, 6510 transcription factors and 4420 EST derived simple sequence repeat (SSR) markers were also predicted. The present study enlightened various characteristic features of P. emblica genome, and provided an important resource for future molecular and functional genomics studies.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 24%
Student > Bachelor 5 17%
Researcher 5 17%
Lecturer 3 10%
Professor 2 7%
Other 4 14%
Unknown 3 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 48%
Biochemistry, Genetics and Molecular Biology 3 10%
Engineering 2 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Nursing and Health Professions 1 3%
Other 3 10%
Unknown 5 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 November 2016.
All research outputs
#14,278,154
of 22,896,955 outputs
Outputs from Frontiers in Plant Science
#8,191
of 20,310 outputs
Outputs of similar age
#177,886
of 314,207 outputs
Outputs of similar age from Frontiers in Plant Science
#128
of 416 outputs
Altmetric has tracked 22,896,955 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,310 research outputs from this source. They receive a mean Attention Score of 4.0. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 314,207 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 416 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.