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A Comprehensive and Effective Mass Spectrometry-Based Screening Strategy for Discovery and Identification of New Brassinosteroids from Rice Tissues

Overview of attention for article published in Frontiers in Plant Science, November 2016
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Title
A Comprehensive and Effective Mass Spectrometry-Based Screening Strategy for Discovery and Identification of New Brassinosteroids from Rice Tissues
Published in
Frontiers in Plant Science, November 2016
DOI 10.3389/fpls.2016.01786
Pubmed ID
Authors

Peiyong Xin, Jijun Yan, Bingbing Li, Shuang Fang, Jinshi Fan, Hailong Tian, Yong Shi, Weisheng Tian, Cunyu Yan, Jinfang Chu

Abstract

The exploration and identification of new brassinosteroid (BR) compounds is critical to improve the biosynthetic research of BRs and expand the chemodiversity of active BRs. However, traditional methods are labor-intensive, time-consuming, and less sensitive. Here, we present a facile screening strategy for discovering and identifying novel BRs from plant tissues based on ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). A total of 14 potential BRs were discovered from only 1 g of rice tissues and structurally elucidated by following a MS-based clue, acquired through multiple reaction monitoring (MRM) data-dependent enhanced product ion (EPI) scan, high resolution MS, and MS survey-dependent MS/MS. One of the 14 candidates was identified as 6-deoxo-28-homotyphasterol, a brand new BR compound that is reported for the first time in the BRs biosynthesis pathway. Detailed comparison with reference standards and quantitative level analysis in rice BR mutants confirmed the availability of the other candidates. This effective, yet simple method provides an efficient way to find more and more chemically new BR biosynthetic intermediates in plants, which is significant for complementing the biosynthesis and metabolism network of BRs. This strategy may also be used to discover unknown compounds of other plant hormone species as well as their key metabolites.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 25%
Student > Bachelor 4 14%
Professor > Associate Professor 3 11%
Student > Postgraduate 2 7%
Student > Doctoral Student 1 4%
Other 4 14%
Unknown 7 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 25%
Chemistry 5 18%
Engineering 3 11%
Biochemistry, Genetics and Molecular Biology 2 7%
Computer Science 1 4%
Other 1 4%
Unknown 9 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 December 2016.
All research outputs
#18,345,176
of 23,567,572 outputs
Outputs from Frontiers in Plant Science
#12,807
of 21,641 outputs
Outputs of similar age
#291,319
of 419,193 outputs
Outputs of similar age from Frontiers in Plant Science
#275
of 495 outputs
Altmetric has tracked 23,567,572 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 21,641 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 419,193 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 495 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.