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Function Identification of the Nucleotides in Key cis-Element of DYSFUNCTIONAL TAPETUM1 (DYT1) Promoter

Overview of attention for article published in Frontiers in Plant Science, February 2017
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Title
Function Identification of the Nucleotides in Key cis-Element of DYSFUNCTIONAL TAPETUM1 (DYT1) Promoter
Published in
Frontiers in Plant Science, February 2017
DOI 10.3389/fpls.2017.00153
Pubmed ID
Authors

Shumin Zhou, Hongli Zhang, Ruisha Li, Qiang Hong, Yang Li, Qunfang Xia, Wei Zhang

Abstract

As a core regulatory gene of the anther development, DYSFUNCTIONAL TAPETUM1 (DYT1) was expressed in tapetum preferentially. Previous study had confirmed that a "CTCC" sequence within DYT1 promoter was indispensable for correct DYT1 expression. However, precise analysis on the function of each nucleotide of this sequence still lacks. Here we employed site mutation assay to identify the function roles of the nucleotides. As a result, the "T" and final "C" of "CTCC" were found essential for the temporal and spatial specificity of DYT1 expression, whereas the other two "C" nucleotides exhibited substitutable somewhat. The substitutes of two flanking nucleotides of "CTCC," however, hardly affected the normal promoter function, suggesting that the "CTCC" sequence as a whole did meet the standard of a canonical cis-element by definition. In addition, it was found that as short as 497 bp DYT1 promoter was sufficient for tissue-specific expression, while longer 505 bp DYT1 promoter sequence was sufficient for species-specific expression.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 18%
Student > Ph. D. Student 3 18%
Researcher 2 12%
Other 1 6%
Lecturer > Senior Lecturer 1 6%
Other 2 12%
Unknown 5 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 29%
Agricultural and Biological Sciences 5 29%
Psychology 1 6%
Design 1 6%
Unknown 5 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2017.
All research outputs
#20,411,380
of 22,961,203 outputs
Outputs from Frontiers in Plant Science
#16,284
of 20,389 outputs
Outputs of similar age
#269,898
of 309,432 outputs
Outputs of similar age from Frontiers in Plant Science
#396
of 513 outputs
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