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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Vacuum and Co-cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize
|
|---|---|
| Published in |
Frontiers in Plant Science, March 2017
|
| DOI | 10.3389/fpls.2017.00393 |
| Pubmed ID | |
| Authors |
Ju Zhang, Deshui Yu, Yi Zhang, Kun Liu, Kedong Xu, Fuli Zhang, Jian Wang, Guangxuan Tan, Xianhui Nie, Qiaohua Ji, Lu Zhao, Chengwei Li |
| Abstract |
Tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) has been frequently used in dicots. Here we show that it can also be used in monocots, by presenting a system involving use of a novel infiltration solution (containing acetosyringone, cysteine, and Tween 20) that enables whole-plant level VIGS of (germinated) seeds in wheat and maize. Using the established system, phytoene desaturase (PDS) genes were successfully silenced, resulting in typical photo-bleaching symptoms in the leaves of treated wheat and maize. In addition, three wheat homoeoalleles of MLO, a key gene repressing defense responses to powdery mildew in wheat, were simultaneously silenced in susceptible wheat with this system, resulting in it becoming resistant to powdery mildew. The system has the advantages generally associated with TRV-mediated VIGS systems (e.g., high-efficiency, mild virus infection symptoms, and effectiveness in different organs). However, it also has the following further advantages: (germinated) seed-stage agroinfiltration; greater rapidity and convenience; whole-plant level gene silencing; adequately stable transformation; and suitability for studying functions of genes involved in seed germination and early plant development stages. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| New Zealand | 1 | 13% |
| Canada | 1 | 13% |
| United Kingdom | 1 | 13% |
| Unknown | 5 | 63% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 75% |
| Scientists | 2 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 139 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| Unknown | 138 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 30 | 22% |
| Student > Master | 22 | 16% |
| Researcher | 16 | 12% |
| Student > Doctoral Student | 11 | 8% |
| Student > Bachelor | 8 | 6% |
| Other | 21 | 15% |
| Unknown | 31 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 65 | 47% |
| Biochemistry, Genetics and Molecular Biology | 32 | 23% |
| Engineering | 2 | 1% |
| Business, Management and Accounting | 1 | <1% |
| Computer Science | 1 | <1% |
| Other | 3 | 2% |
| Unknown | 35 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 April 2017.
All research outputs
#6,423,708
of 22,961,203 outputs
Outputs from Frontiers in Plant Science
#3,639
of 20,389 outputs
Outputs of similar age
#104,215
of 309,336 outputs
Outputs of similar age from Frontiers in Plant Science
#101
of 538 outputs
Altmetric has tracked 22,961,203 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 20,389 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 309,336 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 538 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.