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Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L

Overview of attention for article published in Frontiers in Plant Science, June 2017
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Title
Quantitative Trait Locus Mapping of Salt Tolerance and Identification of Salt-Tolerant Genes in Brassica napus L
Published in
Frontiers in Plant Science, June 2017
DOI 10.3389/fpls.2017.01000
Pubmed ID
Authors

Lina Lang, Aixia Xu, Juan Ding, Yan Zhang, Na Zhao, Zhengshu Tian, Yaping Liu, Yang Wang, Xia Liu, Fenghao Liang, Bingbing Zhang, Mengfan Qin, Jazira Dalelhan, Zhen Huang

Abstract

Salinity stress is one of typical abiotic stresses that seriously limit crop production. In this study, a genetic linkage map based on 532 molecular markers covering 1341.1 cM was constructed to identify the loci associated with salt tolerance in Brassica napus. Up to 45 quantitative trait loci (QTLs) for 10 indicators were identified in the F2:3 populations. These QTLs can account for 4.80-51.14% of the phenotypic variation. A major QTL, qSPAD5 on LG5 associated with chlorophyll can be detected in three replicates. Two intron polymorphic (IP) markers in this QTL region were developed successfully to narrow down the QTL location to a region of 390 kb. A salt tolerance related gene Bra003640 was primary identified as the candidate gene in this region. The full length of the candidate gene was 1,063 bp containing three exons and two introns in B. napus L. The open reading frame (ORF) is 867 bp and encodes 287 amino acids. Three amino acid differences (34, 54, and 83) in the conserved domain (B-box) were identified. RT-qPCR analysis showed that the gene expression had significant difference between the two parents. The study laid great foundation for salt tolerance related gene mapping and cloning in B. napus L.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 21%
Student > Doctoral Student 4 14%
Researcher 2 7%
Student > Bachelor 1 3%
Student > Master 1 3%
Other 3 10%
Unknown 12 41%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 45%
Biochemistry, Genetics and Molecular Biology 3 10%
Medicine and Dentistry 1 3%
Unknown 12 41%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 June 2017.
All research outputs
#20,431,953
of 22,985,065 outputs
Outputs from Frontiers in Plant Science
#16,329
of 20,435 outputs
Outputs of similar age
#276,265
of 317,518 outputs
Outputs of similar age from Frontiers in Plant Science
#502
of 575 outputs
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