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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule
|
|---|---|
| Published in |
Frontiers in Plant Science, August 2017
|
| DOI | 10.3389/fpls.2017.01441 |
| Pubmed ID | |
| Authors |
Haroon Butt, Ayman Eid, Zahir Ali, Mohamed A. M. Atia, Morad M. Mokhtar, Norhan Hassan, Ciaran M. Lee, Gang Bao, Magdy M. Mahfouz |
| Abstract |
The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous delivery of a DNA template for homology-directed repair (HDR). Here, we used CRISPR/Cas9 to generate targeted double-strand breaks and to deliver an RNA repair template for HDR in rice (Oryza sativa). We used chimeric single-guide RNA (cgRNA) molecules carrying both sequences for target site specificity (to generate the double-strand breaks) and repair template sequences (to direct HDR), flanked by regions of homology to the target. Gene editing was more efficient in rice protoplasts using repair templates complementary to the non-target DNA strand, rather than the target strand. We applied this cgRNA repair method to generate herbicide resistance in rice, which showed that this cgRNA repair method can be used for targeted gene editing in plants. Our findings will facilitate applications in functional genomics and targeted improvement of crop traits. |
X Demographics
The data shown below were collected from the profiles of 19 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 11% |
| Spain | 2 | 11% |
| Germany | 1 | 5% |
| San Marino | 1 | 5% |
| Mexico | 1 | 5% |
| United States | 1 | 5% |
| Brazil | 1 | 5% |
| Switzerland | 1 | 5% |
| Pakistan | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 8 | 42% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 14 | 74% |
| Scientists | 5 | 26% |
Mendeley readers
The data shown below were compiled from readership statistics for 200 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 200 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 39 | 20% |
| Student > Ph. D. Student | 27 | 14% |
| Student > Bachelor | 20 | 10% |
| Student > Master | 18 | 9% |
| Other | 9 | 5% |
| Other | 25 | 13% |
| Unknown | 62 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 63 | 32% |
| Biochemistry, Genetics and Molecular Biology | 51 | 26% |
| Immunology and Microbiology | 3 | 2% |
| Engineering | 3 | 2% |
| Unspecified | 2 | 1% |
| Other | 12 | 6% |
| Unknown | 66 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 26. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2024.
All research outputs
#1,498,507
of 25,734,859 outputs
Outputs from Frontiers in Plant Science
#466
of 24,922 outputs
Outputs of similar age
#28,898
of 325,925 outputs
Outputs of similar age from Frontiers in Plant Science
#8
of 490 outputs
Altmetric has tracked 25,734,859 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 24,922 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 325,925 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 490 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.