Title |
Unravelling Protein-Protein Interaction Networks Linked to Aliphatic and Indole Glucosinolate Biosynthetic Pathways in Arabidopsis
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Published in |
Frontiers in Plant Science, November 2017
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DOI | 10.3389/fpls.2017.02028 |
Pubmed ID | |
Authors |
Sebastian J. Nintemann, Daniel Vik, Julia Svozil, Michael Bak, Katja Baerenfaller, Meike Burow, Barbara A. Halkier |
Abstract |
Within the cell, biosynthetic pathways are embedded in protein-protein interaction networks. In Arabidopsis, the biosynthetic pathways of aliphatic and indole glucosinolate defense compounds are well-characterized. However, little is known about the spatial orchestration of these enzymes and their interplay with the cellular environment. To address these aspects, we applied two complementary, untargeted approaches-split-ubiquitin yeast 2-hybrid and co-immunoprecipitation screens-to identify proteins interacting with CYP83A1 and CYP83B1, two homologous enzymes specific for aliphatic and indole glucosinolate biosynthesis, respectively. Our analyses reveal distinct functional networks with substantial interconnection among the identified interactors for both pathway-specific markers, and add to our knowledge about how biochemical pathways are connected to cellular processes. Specifically, a group of protein interactors involved in cell death and the hypersensitive response provides a potential link between the glucosinolate defense compounds and defense against biotrophic pathogens, mediated by protein-protein interactions. |
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United States | 2 | 50% |
Switzerland | 1 | 25% |
United Kingdom | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 45 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 11 | 24% |
Student > Master | 11 | 24% |
Researcher | 9 | 20% |
Student > Doctoral Student | 4 | 9% |
Professor > Associate Professor | 2 | 4% |
Other | 3 | 7% |
Unknown | 5 | 11% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 18 | 40% |
Biochemistry, Genetics and Molecular Biology | 15 | 33% |
Chemistry | 3 | 7% |
Computer Science | 1 | 2% |
Environmental Science | 1 | 2% |
Other | 0 | 0% |
Unknown | 7 | 16% |