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Mendeley readers
Attention Score in Context
| Title |
Induction of Terpene Biosynthesis in Berries of Microvine Transformed with VvDXS1 Alleles
|
|---|---|
| Published in |
Frontiers in Plant Science, January 2018
|
| DOI | 10.3389/fpls.2017.02244 |
| Pubmed ID | |
| Authors |
Lorenza Dalla Costa, Francesco Emanuelli, Massimiliano Trenti, Paula Moreno-Sanz, Silvia Lorenzi, Emanuela Coller, Sergio Moser, Davide Slaghenaufi, Alessandro Cestaro, Roberto Larcher, Ivana Gribaudo, Laura Costantini, Mickael Malnoy, M. Stella Grando |
| Abstract |
Terpenoids, especially monoterpenes, are major aroma-impact compounds in grape and wine. Previous studies highlighted a key regulatory role for grapevine 1-deoxy-D-xylulose 5-phosphate synthase 1 (VvDXS1), the first enzyme of the methylerythritol phosphate pathway for isoprenoid precursor biosynthesis. Here, the parallel analysis of VvDXS1 genotype and terpene concentration in a germplasm collection demonstrated that VvDXS1 sequence has a very high predictive value for the accumulation of monoterpenes and also has an influence on sesquiterpene levels. A metabolic engineering approach was applied by expressing distinct VvDXS1 alleles in the grapevine model system "microvine" and assessing the effects on downstream pathways at transcriptional and metabolic level in different organs and fruit developmental stages. The underlying goal was to investigate two potential perturbation mechanisms, the former based on a significant over-expression of the wild-type (neutral) VvDXS1 allele and the latter on the ex-novo expression of an enzyme with increased catalytic efficiency from the mutated (muscat) VvDXS1 allele. The integration of the two VvDXS1 alleles in distinct microvine lines was found to alter the expression of several terpenoid biosynthetic genes, as assayed through an ad hoc developed TaqMan array based on cDNA libraries of four aromatic cultivars. In particular, enhanced transcription of monoterpene, sesquiterpene and carotenoid pathway genes was observed. The accumulation of monoterpenes in ripe berries was higher in the transformed microvines compared to control plants. This effect is predominantly attributed to the improved activity of the VvDXS1 enzyme coded by the muscat allele, whereas the up-regulation of VvDXS1 plays a secondary role in the increase of monoterpenes. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 70 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 16% |
| Researcher | 10 | 14% |
| Student > Master | 10 | 14% |
| Student > Doctoral Student | 7 | 10% |
| Student > Postgraduate | 5 | 7% |
| Other | 7 | 10% |
| Unknown | 20 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 31 | 44% |
| Biochemistry, Genetics and Molecular Biology | 9 | 13% |
| Chemistry | 2 | 3% |
| Business, Management and Accounting | 1 | 1% |
| Computer Science | 1 | 1% |
| Other | 3 | 4% |
| Unknown | 23 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2018.
All research outputs
#20,465,050
of 23,023,224 outputs
Outputs from Frontiers in Plant Science
#16,427
of 20,547 outputs
Outputs of similar age
#378,741
of 441,902 outputs
Outputs of similar age from Frontiers in Plant Science
#386
of 448 outputs
Altmetric has tracked 23,023,224 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,547 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 448 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.