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Mendeley readers
Attention Score in Context
| Title |
Robust Microplate-Based Methods for Culturing and in Vivo Phenotypic Screening of Chlamydomonas reinhardtii
|
|---|---|
| Published in |
Frontiers in Plant Science, March 2018
|
| DOI | 10.3389/fpls.2018.00235 |
| Pubmed ID | |
| Authors |
Timothy C. Haire, Cody Bell, Kirstin Cutshaw, Brendan Swiger, Kurt Winkelmann, Andrew G. Palmer |
| Abstract |
Chlamydomonas reinhardtii (Cr), a unicellular alga, is routinely utilized to study photosynthetic biochemistry, ciliary motility, and cellular reproduction. Its minimal culture requirements, unicellular morphology, and ease of transformation have made it a popular model system. Despite its relatively slow doubling time, compared with many bacteria, it is an ideal eukaryotic system for microplate-based studies utilizing either, or both, absorbance as well as fluorescence assays. Such microplate assays are powerful tools for researchers in the areas of toxicology, pharmacology, chemical genetics, biotechnology, and more. However, while microplate-based assays are valuable tools for screening biological systems, these methodologies can significantly alter the conditions in which the organisms are cultured and their subsequent physiology or morphology. Herein we describe a novel method for the microplate culture and in vivo phenotypic analysis of growth, viability, and photosynthetic pigments of C. reinhardtii. We evaluated the utility of our assay by screening silver nanoparticles for their effects on growth and viability. These methods are amenable to a wide assortment of studies and present a significant advancement in the methodologies available for research involving this model organism. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 85 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 26% |
| Student > Bachelor | 10 | 12% |
| Student > Master | 9 | 11% |
| Researcher | 8 | 9% |
| Student > Doctoral Student | 7 | 8% |
| Other | 9 | 11% |
| Unknown | 20 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 22 | 26% |
| Agricultural and Biological Sciences | 22 | 26% |
| Environmental Science | 4 | 5% |
| Immunology and Microbiology | 4 | 5% |
| Earth and Planetary Sciences | 2 | 2% |
| Other | 8 | 9% |
| Unknown | 23 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2018.
All research outputs
#18,604,390
of 23,045,021 outputs
Outputs from Frontiers in Plant Science
#14,036
of 20,607 outputs
Outputs of similar age
#258,261
of 332,516 outputs
Outputs of similar age from Frontiers in Plant Science
#369
of 466 outputs
Altmetric has tracked 23,045,021 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,607 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 332,516 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 466 others from the same source and published within six weeks on either side of this one. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.