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Attention Score in Context
| Title |
The Novel Secreted Meloidogyne incognita Effector MiISE6 Targets the Host Nucleus and Facilitates Parasitism in Arabidopsis
|
|---|---|
| Published in |
Frontiers in Plant Science, March 2018
|
| DOI | 10.3389/fpls.2018.00252 |
| Pubmed ID | |
| Authors |
Qianqian Shi, Zhenchuan Mao, Xiaoping Zhang, Jian Ling, Runmao Lin, Xi Zhang, Rui Liu, Yunsheng Wang, Yuhong Yang, Xinyue Cheng, Bingyan Xie |
| Abstract |
Meloidogyne incognita is highly specialized parasite that interacts with host plants using a range of strategies. The effectors are synthesized in the esophageal glands and secreted into plant cells through a needle-like stylet during parasitism. In this study, based on RNA-seq and bioinformatics analysis, we predicted 110 putativeMeloidogyne incognitaeffectors that contain nuclear localization signals (NLSs). Combining theBurkholderia glumae-pEDV based screening system with subcellular localization, from 20 randomly selected NLS effector candidates, we identified an effector MiISE6 that can effectively suppressB. glumae-induced cell death inNicotiana benthamiana, targets to the nuclei of plant cells, and is highly expressed in early parasitic J2 stage. Sequence analysis showed that MiISE6 is a 157-amino acid peptide, with an OGFr_N domain and two NLS motifs. Hybridizationin situverified thatMiISE6is expressed in the subventral esophageal glands. Yeast invertase secretion assay validated the function of the signal peptide harbored in MiISE6. TransgenicArabidopsis thalianaplants expressingMiISE6become more susceptible toM. incognita. Inversely, the host-derived RNAi ofMiISE6of the nematode can decrease its parasitism on host. Based on transcriptome analysis of theMiISE6transgenicArabidopsissamples and the wild-type samples, we obtained 852 differentially expressed genes (DEGs). Integrating Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, we found that expression ofMiISE6inArabidopsiscan suppress jasmonate signaling pathway. In addition, the expression of genes related to cell wall modification and the ubiquitination proteasome pathway also have detectable changes in the transgenic plants. Results from the present study suggest thatMiISE6is involved in interaction between nematode-plant, and plays an important role during the early stages of parasitism by interfering multiple signaling pathways of plant. Moreover, we found homologs of MiISE6 in other sedentary nematodes,Meloidogyne haplaandGlobodera pallida. Our experimental results provide evidence to decipher the molecular mechanisms underlying the manipulation of host immune defense responses by plant parasitic nematodes, and transcriptome data also provide useful information for further study nematode-plant interactions. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 14% |
| United Kingdom | 1 | 14% |
| Switzerland | 1 | 14% |
| France | 1 | 14% |
| Unknown | 3 | 43% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 57% |
| Scientists | 2 | 29% |
| Science communicators (journalists, bloggers, editors) | 1 | 14% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 52 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 17% |
| Student > Master | 9 | 17% |
| Researcher | 8 | 15% |
| Student > Bachelor | 3 | 6% |
| Student > Doctoral Student | 3 | 6% |
| Other | 8 | 15% |
| Unknown | 12 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 25 | 48% |
| Biochemistry, Genetics and Molecular Biology | 7 | 13% |
| Engineering | 2 | 4% |
| Business, Management and Accounting | 1 | 2% |
| Materials Science | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 15 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 April 2018.
All research outputs
#13,075,788
of 23,041,514 outputs
Outputs from Frontiers in Plant Science
#5,677
of 20,598 outputs
Outputs of similar age
#160,880
of 331,463 outputs
Outputs of similar age from Frontiers in Plant Science
#183
of 464 outputs
Altmetric has tracked 23,041,514 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,598 research outputs from this source. They receive a mean Attention Score of 4.0. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,463 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 464 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.