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Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

Overview of attention for article published in Frontiers in Plant Science, June 2018
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Title
Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum
Published in
Frontiers in Plant Science, June 2018
DOI 10.3389/fpls.2018.00740
Pubmed ID
Authors

Daniel Stukenberg, Stefan Zauner, Gianluca Dell’Aquila, Uwe G. Maier

Abstract

CRISPR/Cas9 is a powerful tool for genome editing. We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. In our experiments, we targeted two different genes, screened for mutations and analyzed mutated diatoms in a three-step process. In the end, we identified cells, showing either monoallelic or homo-biallelic targeted mutations. Thus, we confirm that application of the CRISPR/Cas9 system for P. tricornutum is very promising, although, as discussed, overlooked pitfalls have to be considered.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 128 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 128 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 27 21%
Researcher 17 13%
Student > Master 17 13%
Student > Bachelor 11 9%
Student > Postgraduate 6 5%
Other 12 9%
Unknown 38 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 39 30%
Agricultural and Biological Sciences 29 23%
Medicine and Dentistry 2 2%
Environmental Science 2 2%
Chemical Engineering 2 2%
Other 9 7%
Unknown 45 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 May 2018.
All research outputs
#17,962,718
of 23,067,276 outputs
Outputs from Frontiers in Plant Science
#12,261
of 20,656 outputs
Outputs of similar age
#238,102
of 329,290 outputs
Outputs of similar age from Frontiers in Plant Science
#319
of 476 outputs
Altmetric has tracked 23,067,276 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 20,656 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 329,290 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 476 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.