Title |
Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum
|
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Published in |
Frontiers in Plant Science, June 2018
|
DOI | 10.3389/fpls.2018.00740 |
Pubmed ID | |
Authors |
Daniel Stukenberg, Stefan Zauner, Gianluca Dell’Aquila, Uwe G. Maier |
Abstract |
CRISPR/Cas9 is a powerful tool for genome editing. We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. In our experiments, we targeted two different genes, screened for mutations and analyzed mutated diatoms in a three-step process. In the end, we identified cells, showing either monoallelic or homo-biallelic targeted mutations. Thus, we confirm that application of the CRISPR/Cas9 system for P. tricornutum is very promising, although, as discussed, overlooked pitfalls have to be considered. |
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Geographical breakdown
Country | Count | As % |
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Belgium | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 128 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 27 | 21% |
Researcher | 17 | 13% |
Student > Master | 17 | 13% |
Student > Bachelor | 11 | 9% |
Student > Postgraduate | 6 | 5% |
Other | 12 | 9% |
Unknown | 38 | 30% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 39 | 30% |
Agricultural and Biological Sciences | 29 | 23% |
Medicine and Dentistry | 2 | 2% |
Environmental Science | 2 | 2% |
Chemical Engineering | 2 | 2% |
Other | 9 | 7% |
Unknown | 45 | 35% |