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Three-Dimensional Reconstruction and Segmentation of Intact Drosophila by Ultramicroscopy

Overview of attention for article published in Frontiers in Systems Neuroscience, January 2010
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  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

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119 Mendeley
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Title
Three-Dimensional Reconstruction and Segmentation of Intact Drosophila by Ultramicroscopy
Published in
Frontiers in Systems Neuroscience, January 2010
DOI 10.3389/neuro.06.001.2010
Pubmed ID
Authors

Nina Jährling, Klaus Becker, Cornelia Schönbauer, Frank Schnorrer, Hans-Ulrich Dodt

Abstract

Genetic mutants are invaluable for understanding the development, physiology and behaviour of Drosophila. Modern molecular genetic techniques enable the rapid generation of large numbers of different mutants. To phenotype these mutants sophisticated microscopy techniques are required, ideally allowing the 3D-reconstruction of the anatomy of an adult fly from a single scan. Ultramicroscopy enables up to cm fields of view, whilst providing micron resolution. In this paper, we present ultramicroscopy reconstructions of the flight musculature, the nervous system, and the digestive tract of entire, chemically cleared, drosophila in autofluorescent light. The 3D-reconstructions thus obtained verify that the anatomy of a whole fly, including the filigree spatial organization of the direct flight muscles, can be analysed from a single ultramicroscopy reconstruction. The recording procedure, including 3D-reconstruction using standard software, takes no longer than 30 min. Additionally, image segmentation, which would allow for further quantitative analysis, was performed.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 119 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 3 3%
Austria 2 2%
Portugal 1 <1%
Switzerland 1 <1%
France 1 <1%
Italy 1 <1%
Colombia 1 <1%
Czechia 1 <1%
United Kingdom 1 <1%
Other 2 2%
Unknown 105 88%

Demographic breakdown

Readers by professional status Count As %
Researcher 40 34%
Student > Ph. D. Student 22 18%
Student > Master 11 9%
Professor > Associate Professor 8 7%
Student > Bachelor 7 6%
Other 18 15%
Unknown 13 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 47 39%
Biochemistry, Genetics and Molecular Biology 15 13%
Engineering 10 8%
Neuroscience 9 8%
Physics and Astronomy 8 7%
Other 17 14%
Unknown 13 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 June 2021.
All research outputs
#7,454,951
of 22,790,780 outputs
Outputs from Frontiers in Systems Neuroscience
#606
of 1,342 outputs
Outputs of similar age
#48,416
of 163,926 outputs
Outputs of similar age from Frontiers in Systems Neuroscience
#7
of 23 outputs
Altmetric has tracked 22,790,780 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,342 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.6. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 163,926 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.