Physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed supplement

Physico-chemical properties of late-incubation egg amniotic fluid and a potential <i>in ovo</i> feed supplement

Animal Bioscience, January 2017

28183170

A. A. Omede, M. M. Bhuiyan, A. F. lslam, P. A. Iji

This study explored the physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed (IOF) supplement. Amniotic fluid was collected from broiler breeders (Ross 308, 51 weeks and Cobb 500, 35 weeks) on day 17 after incubation. A mixture of high-quality soy protein supplement - Hamlet Protein AviStart (HPA) was serially diluted in MilliQ water to obtain solutions ranging from 150 to 9.375 mg/ml. The mixtures were heat-treated (0, 30, 60 minutes) in a waterbath (80 oC) and then centrifuged to obtain supernatants. The amniotic fluid and HPA supernatants were analysed for their physico-chemical properties. Only viscosity and K+ were significantly (P < 0.05) different in both strains. Of all essential amino acids, leucine and lysine were in the highest concentration in both strains. The osmolality, viscosity and pCO2 of the supernatants decreased (P < 0.05) with decreasing HPA concentration. Heat treatment significantly (P < 0.05) affected osmolality, pH, and pCO2, of the supernatants. The interactions between HPA concentration and heat treatment were significant with regards to osmolality (P < 0.01), pH (P < 0.01), pCO2 (P < 0.05), glucose (P < 0.05), lactate (P < 0.01) and acid-base status (P < 0.01) of HPA solutions. The Ca2+, K+, glucose, and lactate increased with increasing concentration of HPA solution. The protein content of HPA solutions decreased (P < 0.05) with reduced HPA solution concentrations. The supernatant from 150 mg/ml HPA solution was richest in glutamic acid, aspartic acid, arginine and lysine. Amino acids concentrations were reduced (P < 0.05) with each serial dilution but increased with longer heating. The values obtained in the primary solution (highest concentration) are close to the profiles of high-protein ingredients. This supplement, as a solution, hence, may be suitable for use as an IOF supplement and should be tested for this potential.