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X Demographics
Mendeley readers
Attention Score in Context
| Title |
PCR-RFLPによるツキヨタケの迅速判別法
|
|---|---|
| Published in |
Shokuhin eiseigaku zasshi Journal of the Food Hygienic Society of Japan, January 2017
|
| DOI | 10.3358/shokueishi.58.113 |
| Pubmed ID | |
| Authors |
Yohei Sugano, Kozue Sakata, Kosuke Nakamura, Akio Noguchi, Nozomi Fukuda, Tomohiro Suzuki, Kazunari Kondo |
| Abstract |
Omphalotus japonicus is a poisonous mushroom that grows in Japan. It can be mistaken for edible mushrooms (Shiitake, Hiratake and Mukitake), and if ingested, it causes food poisoning within 30 min to 1 hr. We established a rapid detection method using PCR-RFLP to identify O. japonicus by restriction digestion of the amplified ITS region. By using Sau96I, Bpu10I, SfcI or DrdI/HincII as a restriction enzyme, it was possible to rapidly identify and discriminate O. japonicus based on the fragment length. This study also provided a short PCR-RFLP system comprising amplification and digestion of a short 200-bp DNA fragment within the ITS region. The system could identify and discriminate O. japonicus after in vitro gastric digestion of native and heated mushroom samples as a model of food poisoning. In addition, a confirmatory assay using real-time PCR was developed to achieve more sensitive detection of O. japonicus. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 2 | 29% |
| Researcher | 2 | 29% |
| Student > Bachelor | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 29% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Nursing and Health Professions | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Unknown | 2 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 October 2019.
All research outputs
#6,446,325
of 25,382,440 outputs
Outputs from Shokuhin eiseigaku zasshi Journal of the Food Hygienic Society of Japan
#77
of 521 outputs
Outputs of similar age
#108,602
of 421,709 outputs
Outputs of similar age from Shokuhin eiseigaku zasshi Journal of the Food Hygienic Society of Japan
#3
of 16 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 521 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,709 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.