| Title |
Lipophilic components of the brown seaweed, Ascophyllum nodosum, enhance freezing tolerance in Arabidopsis thaliana
|
|---|---|
| Published in |
Planta, April 2009
|
| DOI | 10.1007/s00425-009-0920-8 |
| Pubmed ID | |
| Authors |
Prasanth Rayirath, Bernhard Benkel, D. Mark Hodges, Paula Allan-Wojtas, Shawna MacKinnon, Alan T. Critchley, Balakrishnan Prithiviraj |
| Abstract |
Extracts of the brown seaweed Ascophyllum nodosum enhance plant tolerance against environmental stresses such as drought, salinity, and frost. However, the molecular mechanisms underlying this improved stress tolerance and the nature of the bioactive compounds present in the seaweed extracts that elicits stress tolerance remain largely unknown. We investigated the effect of A. nodosum extracts and its organic sub-fractions on freezing tolerance of Arabidopsis thaliana. Ascophyllum nodosum extracts and its lipophilic fraction significantly increased tolerance to freezing temperatures in in vitro and in vivo assays. Untreated plants exhibited severe chlorosis, tissue damage, and failed to recover from freezing treatments while the extract-treated plants recovered from freezing temperature of -7.5 degrees C in in vitro and -5.5 degrees C in in vivo assays. Electrolyte leakage measurements revealed that the LT(50) value was lowered by 3 degrees C while cell viability staining demonstrated a 30-40% reduction in area of damaged tissue in extract treated plants as compared to water controls. Moreover, histological observations of leaf sections revealed that extracts have a significant effect on maintaining membrane integrity during freezing stress. Treated plants exhibited 70% less chlorophyll damage during freezing recovery as compared to the controls, and this correlated with reduced expression of the chlorphyllase genes AtCHL1 and AtCHL2. Further, the A. nodosum extract treatment modulated the expression of the cold response genes, COR15A, RD29A, and CBF3, resulting in enhanced tolerance to freezing temperatures. More than 2.6-fold increase in expression of RD29A, 1.8-fold increase of CBF3 and two-fold increase in the transcript level of COR15A was observed in plants treated with lipophilic fraction of A. nodosum at -2 degrees C. Taken together, the results suggest that chemical components in A. nodosum extracts protect membrane integrity and affect the expression of stress response genes leading to freezing stress tolerance in A. thaliana. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 178 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 2 | 1% |
| Brazil | 1 | <1% |
| Sweden | 1 | <1% |
| Korea, Republic of | 1 | <1% |
| Spain | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 171 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 38 | 21% |
| Student > Ph. D. Student | 35 | 20% |
| Student > Bachelor | 21 | 12% |
| Student > Master | 19 | 11% |
| Student > Doctoral Student | 9 | 5% |
| Other | 21 | 12% |
| Unknown | 35 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 99 | 56% |
| Biochemistry, Genetics and Molecular Biology | 13 | 7% |
| Environmental Science | 10 | 6% |
| Chemistry | 5 | 3% |
| Unspecified | 2 | 1% |
| Other | 11 | 6% |
| Unknown | 38 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 February 2014.
All research outputs
#15,298,293
of 22,751,628 outputs
Outputs from Planta
#1,865
of 2,718 outputs
Outputs of similar age
#78,914
of 93,497 outputs
Outputs of similar age from Planta
#8
of 9 outputs
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