| Chapter title |
Ferritin encapsulation and templated synthesis of inorganic nanoparticles.
|
|---|---|
| Chapter number | 3 |
| Book title |
Protein Cages
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2131-7_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2130-0, 978-1-4939-2131-7
|
| Authors |
Katherine W Pulsipher, Ivan J Dmochowski, Katherine W. Pulsipher, Ivan J. Dmochowski |
| Abstract |
Understanding how inorganic nanoparticles interact with proteins is paramount to their safe and effective use in vivo. Ordered protein-inorganic nanomaterial assemblies will also enable the creation of patterned structures with useful physical properties. Thermophilic ferritin (tF) from Archaeoglobus fulgidus has unique structural features and self-assembly properties that facilitate stable but also reversible interaction with gold nanoparticles (AuNPs). In this chapter we describe how to express and purify tF and induce its assembly around AuNPs. We also describe methods for characterizing the tF-AuNP complex as well as templating NP growth within the protein cavity. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 45% |
| Professor | 2 | 18% |
| Student > Bachelor | 1 | 9% |
| Student > Doctoral Student | 1 | 9% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 27% |
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Agricultural and Biological Sciences | 2 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
| Unknown | 3 | 27% |