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High Throughput Protein Expression and Purification

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Cover of 'High Throughput Protein Expression and Purification'

Table of Contents

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    Book Overview
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    Chapter 1 High-Throughput Protein Production (HTPP): A Review of Enabling Technologies to Expedite Protein Production
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    Chapter 2 Designing Experiments for High-Throughput Protein Expression
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    Chapter 3 Gateway cloning for protein expression.
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    Chapter 4 Flexi Vector Cloning
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    Chapter 5 The Precise Engineering of Expression Vectors Using High-Throughput In-Fusion™ PCR Cloning
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    Chapter 6 The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis.
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    Chapter 7 A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins
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    Chapter 8 “System 48” High-Throughput Cloning and Protein Expression Analysis
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    Chapter 9 Automated 96-Well Purification of Hexahistidine-Tagged Recombinant Proteins on MagneHis Ni 2 +-Particles
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    Chapter 10 E. coli and Insect Cell Expression, Automated Purification and Quantitative Analysis
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    Chapter 11 Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli.
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    Chapter 12 PHB-Intein-Mediated Protein Purification Strategy
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    Chapter 13 High-throughput biotinylation of proteins.
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    Chapter 14 High-Throughput Insect Cell Protein Expression Applications
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    Chapter 15 High-Throughput Protein Expression Using Cell-Free System
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    Chapter 16 The Production of Glycoproteins by Transient Expression in Mammalian Cells
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    Chapter 17 High-Throughput Expression and Detergent Screening of Integral Membrane Proteins
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    Chapter 18 Cell-Free Expression for Nanolipoprotein Particles: Building a High-Throughput Membrane Protein Solubility Platform
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    Chapter 19 Expression and purification of soluble His(6)-tagged TEV protease.
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    Chapter 20 High-Throughput Protein Concentration and Buffer Exchange: Comparison of Ultrafiltration and Ammonium Sulfate Precipitation
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Title
High Throughput Protein Expression and Purification
Published by
Humana Press, November 2008
DOI 10.1007/978-1-59745-196-3
ISBNs
978-1-58829-879-9, 978-1-59745-196-3
Editors

Sharon A. Doyle

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 94 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 94 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 23 24%
Researcher 15 16%
Student > Master 14 15%
Student > Bachelor 12 13%
Student > Postgraduate 4 4%
Other 7 7%
Unknown 19 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 38 40%
Agricultural and Biological Sciences 15 16%
Chemistry 6 6%
Chemical Engineering 5 5%
Immunology and Microbiology 4 4%
Other 9 10%
Unknown 17 18%