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High Throughput Protein Expression and Purification

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Cover of 'High Throughput Protein Expression and Purification'

Table of Contents

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    Book Overview
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    Chapter 1 High-Throughput Protein Production (HTPP): A Review of Enabling Technologies to Expedite Protein Production
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    Chapter 2 Designing Experiments for High-Throughput Protein Expression
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    Chapter 3 Gateway cloning for protein expression.
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    Chapter 4 Flexi Vector Cloning
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    Chapter 5 The Precise Engineering of Expression Vectors Using High-Throughput In-Fusion™ PCR Cloning
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    Chapter 6 The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis.
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    Chapter 7 A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins
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    Chapter 8 “System 48” High-Throughput Cloning and Protein Expression Analysis
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    Chapter 9 Automated 96-Well Purification of Hexahistidine-Tagged Recombinant Proteins on MagneHis Ni 2 +-Particles
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    Chapter 10 E. coli and Insect Cell Expression, Automated Purification and Quantitative Analysis
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    Chapter 11 Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli.
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    Chapter 12 PHB-Intein-Mediated Protein Purification Strategy
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    Chapter 13 High-throughput biotinylation of proteins.
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    Chapter 14 High-Throughput Insect Cell Protein Expression Applications
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    Chapter 15 High-Throughput Protein Expression Using Cell-Free System
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    Chapter 16 The Production of Glycoproteins by Transient Expression in Mammalian Cells
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    Chapter 17 High-Throughput Expression and Detergent Screening of Integral Membrane Proteins
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    Chapter 18 Cell-Free Expression for Nanolipoprotein Particles: Building a High-Throughput Membrane Protein Solubility Platform
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    Chapter 19 Expression and purification of soluble His(6)-tagged TEV protease.
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    Chapter 20 High-Throughput Protein Concentration and Buffer Exchange: Comparison of Ultrafiltration and Ammonium Sulfate Precipitation
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Chapter title
High-Throughput Protein Expression Using Cell-Free System
Chapter number 15
Book title
High Throughput Protein Expression and Purification
Published by
Humana Press, January 2009
DOI 10.1007/978-1-59745-196-3_15
Book ISBNs
978-1-58829-879-9, 978-1-59745-196-3
Authors

Kalavathy Sitaraman, Deb K. Chatterjee, Sitaraman, Kalavathy, Chatterjee, Deb K.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 6%
United Kingdom 1 2%
France 1 2%
Unknown 44 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 27%
Researcher 8 16%
Other 5 10%
Student > Bachelor 4 8%
Professor 4 8%
Other 11 22%
Unknown 4 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 51%
Biochemistry, Genetics and Molecular Biology 6 12%
Chemistry 3 6%
Engineering 2 4%
Chemical Engineering 1 2%
Other 4 8%
Unknown 8 16%