Bacterial Transcriptional Control

Chapter 1 Mapping the Escherichia coli Transcription Elongation Complex with Exonuclease III

Chapter 2 Purification of Bacterial RNA Polymerase: Tools and Protocols

Chapter 3 Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues

Chapter 4 In vitro and in vivo methodologies for studying the sigma 54-dependent transcription.

Chapter 5 Methods for the Assembly and Analysis of In Vitro Transcription-Coupled-to-Translation Systems.

Chapter 6 Site-Specific Incorporation of Probes into RNA Polymerase by Unnatural-Amino-Acid Mutagenesis and Staudinger–Bertozzi Ligation

Chapter 7 Reconstitution of factor-dependent, promoter proximal pausing in Drosophila nuclear extracts.

Chapter 8 Direct Competition Assay for Transcription Fidelity

Chapter 9 Single-Stranded DNA Aptamers for Functional Probing of Bacterial RNA Polymerase

Chapter 10 Biochemical Analysis of Transcription Termination by RNA Polymerase III from Yeast Saccharomyces cerevisiae.

Chapter 11 Use of RNA Polymerase Molecular Beacon Assay to Measure RNA Polymerase Interactions with Model Promoter Fragments

Chapter 12 Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends.

Chapter 13 In Situ Footprinting of E. coli Transcription Elongation Complex with Chloroacetaldehyde

Chapter 14 Using Solutes and Kinetics to Probe Large Conformational Changes in the Steps of Transcription Initiation

Chapter 15 Manipulating Archaeal Systems to Permit Analyses of Transcription Elongation-Termination Decisions In Vitro

Chapter 16 Purification of active RNA polymerase I from yeast.

Chapter 17 Transcription in Archaea: Preparation of Methanocaldococcus jannaschii Transcription Machinery.

Chapter 18 Transcription in Archaea: In Vitro Transcription Assays for mjRNAP.

Chapter 19 Experimental Analysis of hFACT Action During Pol II Transcription In Vitro

Chapter 20 ChIP-Seq for Genome-Scale Analysis of Bacterial DNA-Binding Proteins.