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Bacterial Transcriptional Control

Overview of attention for book
Cover of 'Bacterial Transcriptional Control'

Table of Contents

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    Book Overview
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    Chapter 1 Mapping the Escherichia coli Transcription Elongation Complex with Exonuclease III
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    Chapter 2 Purification of Bacterial RNA Polymerase: Tools and Protocols
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    Chapter 3 Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues
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    Chapter 4 In vitro and in vivo methodologies for studying the sigma 54-dependent transcription.
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    Chapter 5 Methods for the Assembly and Analysis of In Vitro Transcription-Coupled-to-Translation Systems.
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    Chapter 6 Site-Specific Incorporation of Probes into RNA Polymerase by Unnatural-Amino-Acid Mutagenesis and Staudinger–Bertozzi Ligation
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    Chapter 7 Reconstitution of factor-dependent, promoter proximal pausing in Drosophila nuclear extracts.
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    Chapter 8 Direct Competition Assay for Transcription Fidelity
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    Chapter 9 Single-Stranded DNA Aptamers for Functional Probing of Bacterial RNA Polymerase
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    Chapter 10 Biochemical Analysis of Transcription Termination by RNA Polymerase III from Yeast Saccharomyces cerevisiae.
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    Chapter 11 Use of RNA Polymerase Molecular Beacon Assay to Measure RNA Polymerase Interactions with Model Promoter Fragments
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    Chapter 12 Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends.
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    Chapter 13 In Situ Footprinting of E. coli Transcription Elongation Complex with Chloroacetaldehyde
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    Chapter 14 Using Solutes and Kinetics to Probe Large Conformational Changes in the Steps of Transcription Initiation
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    Chapter 15 Manipulating Archaeal Systems to Permit Analyses of Transcription Elongation-Termination Decisions In Vitro
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    Chapter 16 Purification of active RNA polymerase I from yeast.
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    Chapter 17 Transcription in Archaea: Preparation of Methanocaldococcus jannaschii Transcription Machinery.
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    Chapter 18 Transcription in Archaea: In Vitro Transcription Assays for mjRNAP.
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    Chapter 19 Experimental Analysis of hFACT Action During Pol II Transcription In Vitro
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    Chapter 20 ChIP-Seq for Genome-Scale Analysis of Bacterial DNA-Binding Proteins.
Attention for Chapter 10: Biochemical Analysis of Transcription Termination by RNA Polymerase III from Yeast Saccharomyces cerevisiae.
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Chapter title
Biochemical Analysis of Transcription Termination by RNA Polymerase III from Yeast Saccharomyces cerevisiae.
Chapter number 10
Book title
Bacterial Transcriptional Control
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2392-2_10
Pubmed ID
25665564
Book ISBNs
978-1-4939-2391-5, 978-1-4939-2392-2
Authors

Aneeshkumar G Arimbasseri, Richard J Maraia, Aneeshkumar G. Arimbasseri, Richard J. Maraia, Arimbasseri, Aneeshkumar G., Maraia, Richard J.

Abstract

Eukaryotic RNA polymerase III (pol III) transcribes short noncoding RNA genes such as those encoding tRNAs, 5S rRNA, U6 snRNA, and a few others. As compared to its pol II counterpart, Pol III has several advantages, including the relative simplicity, stability, and more direct connectivity of its transcription machinery. Only two transcription factor complexes, TFIIIB and TFIIIC, are required to faithfully initiate and direct multiple rounds of transcription by pol III. Moreover, in contrast to an intricate multipartite mechanism of pol II termination, pol III termination is extremely simple, responsive to a monopartite signal (oligo T stretch on the nontemplate DNA strand) and mediated by a stably associated termination subcomplex of three integral subunits (Arimbasseri et al. Transcription 4(6), 2013). This makes pol III a valuable model for dissecting intrinsic molecular mechanisms of eukaryotic transcription termination. In this chapter, we provide protocols we adapted to study the biochemistry of transcription termination by S. cerevisiae pol III.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 42%
Researcher 2 17%
Professor 1 8%
Student > Master 1 8%
Professor > Associate Professor 1 8%
Other 0 0%
Unknown 2 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 42%
Agricultural and Biological Sciences 5 42%
Nursing and Health Professions 1 8%
Unknown 1 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 February 2015.
All research outputs
#20,259,335
of 22,789,076 outputs
Outputs from Methods in molecular biology
#9,890
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Outputs of similar age
#295,704
of 352,984 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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