| Title |
Structure of the voltage-gated calcium channel Cav1.1 at 3.6 Å resolution
|
|---|---|
| Published in |
Nature, August 2016
|
| DOI | 10.1038/nature19321 |
| Pubmed ID | |
| Authors |
Jianping Wu, Zhen Yan, Zhangqiang Li, Xingyang Qian, Shan Lu, Mengqiu Dong, Qiang Zhou, Nieng Yan |
| Abstract |
The voltage-gated calcium (Cav) channels convert membrane electrical signals to intracellular Ca(2+)-mediated events. Among the ten subtypes of Cav channel in mammals, Cav1.1 is specified for the excitation-contraction coupling of skeletal muscles. Here we present the cryo-electron microscopy structure of the rabbit Cav1.1 complex at a nominal resolution of 3.6 Å. The inner gate of the ion-conducting α1-subunit is closed and all four voltage-sensing domains adopt an 'up' conformation, suggesting a potentially inactivated state. The extended extracellular loops of the pore domain, which are stabilized by multiple disulfide bonds, form a windowed dome above the selectivity filter. One side of the dome provides the docking site for the α2δ-1-subunit, while the other side may attract cations through its negative surface potential. The intracellular I-II and III-IV linker helices interact with the β1a-subunit and the carboxy-terminal domain of α1, respectively. Classification of the particles yielded two additional reconstructions that reveal pronounced displacement of β1a and adjacent elements in α1. The atomic model of the Cav1.1 complex establishes a foundation for mechanistic understanding of excitation-contraction coupling and provides a three-dimensional template for molecular interpretations of the functions and disease mechanisms of Cav and Nav channels. |
X Demographics
The data shown below were collected from the profiles of 76 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 16 | 21% |
| United Kingdom | 4 | 5% |
| Japan | 3 | 4% |
| France | 3 | 4% |
| Portugal | 1 | 1% |
| Curaçao | 1 | 1% |
| Kenya | 1 | 1% |
| Mexico | 1 | 1% |
| Denmark | 1 | 1% |
| Other | 8 | 11% |
| Unknown | 37 | 49% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 53 | 70% |
| Scientists | 17 | 22% |
| Practitioners (doctors, other healthcare professionals) | 4 | 5% |
| Science communicators (journalists, bloggers, editors) | 2 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 364 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | <1% |
| United States | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 360 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 69 | 19% |
| Researcher | 46 | 13% |
| Student > Bachelor | 43 | 12% |
| Student > Master | 42 | 12% |
| Professor | 21 | 6% |
| Other | 60 | 16% |
| Unknown | 83 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 96 | 26% |
| Agricultural and Biological Sciences | 52 | 14% |
| Neuroscience | 31 | 9% |
| Chemistry | 24 | 7% |
| Medicine and Dentistry | 23 | 6% |
| Other | 49 | 13% |
| Unknown | 89 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 66. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2023.
All research outputs
#657,866
of 25,791,495 outputs
Outputs from Nature
#26,139
of 98,790 outputs
Outputs of similar age
#12,540
of 349,881 outputs
Outputs of similar age from Nature
#514
of 985 outputs
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