Title |
Survival and Evolution of CRISPR–Cas System in Prokaryotes and Its Applications
|
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Published in |
Frontiers in immunology, September 2016
|
DOI | 10.3389/fimmu.2016.00375 |
Pubmed ID | |
Authors |
Muhammad Abu Bakr Shabbir, Haihong Hao, Muhammad Zubair Shabbir, Hafiz Iftikhar Hussain, Zahid Iqbal, Saeed Ahmed, Adeel Sattar, Mujahid Iqbal, Jun Li, Zonghui Yuan |
Abstract |
Prokaryotes have developed numerous innate immune mechanisms in order to fend off bacteriophage or plasmid attack. One of these immune systems is clustered regularly interspaced short palindromic repeats (CRISPR). CRISPR-associated proteins play a key role in survival of prokaryotes against invaders, as these systems cleave DNA of foreign genetic elements. Beyond providing immunity, these systems have significant impact in altering the bacterial physiology in term of its virulence and pathogenicity, as well as evolution. Also, due to their diverse nature of functionality, cas9 endoribonuclease can be easily reprogrammed with the help of guide RNAs, showing unprecedented potential and significance for gene editing in treating genetic diseases. Here, we also discuss the use of NgAgo-gDNA system in genome editing of human cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Switzerland | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 183 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 32 | 17% |
Student > Master | 27 | 15% |
Student > Ph. D. Student | 26 | 14% |
Student > Doctoral Student | 16 | 9% |
Researcher | 16 | 9% |
Other | 23 | 13% |
Unknown | 43 | 23% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 69 | 38% |
Agricultural and Biological Sciences | 26 | 14% |
Immunology and Microbiology | 12 | 7% |
Medicine and Dentistry | 5 | 3% |
Chemistry | 4 | 2% |
Other | 17 | 9% |
Unknown | 50 | 27% |