Title |
Enhanced Fluorescent Assignment of Protein Aggregates by an Oligothiophene–Porphyrin‐Based Amyloid Ligand
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Published in |
Macromolecular Rapid Communications, March 2013
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DOI | 10.1002/marc.201200817 |
Pubmed ID | |
Authors |
Katriann Arja, Daniel Sjölander, Alma Åslund, Stefan Prokop, Frank L. Heppner, Peter Konradsson, Mikael Lindgren, Per Hammarström, K. O. Andreas Åslund, K. Peter R. Nilsson |
Abstract |
Fluorescent probes identifying protein aggregates are of great interest, as deposition of aggregated proteins is associated with many devastating diseases. Here, we report that a fluorescent amyloid ligand composed of two distinct molecular moieties, an amyloidophilic pentameric oligothiophene and a porphyrin, can be utilized for spectral and lifetime imaging assessment of recombinant Aβ 1-42 amyloid fibrils and Aβ deposits in brain tissue sections from a transgenic mouse model with Alzheimer's disease pathology. The enhanced spectral range and distinct lifetime diversity of this novel oligothiophene-porphyrin-based ligand allow a more precise assessment of heterogeneous amyloid morphology compared with the corresponding oligothiophene dye. |
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Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Sweden | 1 | 3% |
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Unknown | 34 | 94% |
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Researcher | 10 | 28% |
Student > Ph. D. Student | 9 | 25% |
Student > Master | 4 | 11% |
Student > Bachelor | 4 | 11% |
Professor > Associate Professor | 2 | 6% |
Other | 2 | 6% |
Unknown | 5 | 14% |
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Other | 5 | 14% |
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