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Spliceosomal Pre-mRNA Splicing

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Cover of 'Spliceosomal Pre-mRNA Splicing'

Table of Contents

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    Book Overview
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    Chapter 1 The Pre-mRNA Splicing Reaction.
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    Chapter 2 Diversity and Evolution of Spliceosomal Systems
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    Chapter 3 Mechanisms of Spliceosomal Assembly
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    Chapter 4 Alternative Pre-mRNA Splicing
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    Chapter 5 Regulation of Alternative Pre-mRNA Splicing.
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    Chapter 6 Introduction to Cotranscriptional RNA Splicing
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    Chapter 7 Chromatin and Splicing
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    Chapter 8 Preparation of splicing competent nuclear extracts.
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    Chapter 9 Preparation of Yeast Whole Cell Splicing Extract
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    Chapter 10 Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase
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    Chapter 11 In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract.
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    Chapter 12 Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract.
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    Chapter 13 In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation
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    Chapter 14 Isolation and Accumulation of Spliceosomal Assembly Intermediates
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    Chapter 15 Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function
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    Chapter 16 Expression and Purification of Splicing Proteins from Mammalian Cells
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    Chapter 17 Single molecule approaches for studying spliceosome assembly and catalysis.
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    Chapter 18 Cell-Based Splicing of Minigenes
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    Chapter 19 Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA
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    Chapter 20 Antisense Methods to Modulate Pre-mRNA Splicing
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    Chapter 21 Using Yeast Genetics to Study Splicing Mechanisms
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    Chapter 22 Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR
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    Chapter 23 Chromatin Immunoprecipitation Approaches to Determine Co-transcriptional Nature of Splicing
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    Chapter 24 Computational approaches to mine publicly available databases.
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    Chapter 25 Approaches to link RNA secondary structures with splicing regulation
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    Chapter 26 Methods to study splicing from high-throughput RNA sequencing data.
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    Chapter 27 Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq.
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    Chapter 28 Predicting Alternative Splicing
Attention for Chapter 26: Methods to study splicing from high-throughput RNA sequencing data.
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