Title |
Characterization and genetic mapping of the β-diketone deficient eceriferum-b barley mutant
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Published in |
Theoretical and Applied Genetics, March 2017
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DOI | 10.1007/s00122-017-2877-5 |
Pubmed ID | |
Authors |
Qin Zhou, Chao Li, Kohei Mishina, Jiecai Zhao, Jiwei Zhang, Ruijun Duan, Xiaoying Ma, Aidong Wang, Qianxiang Meng, Takao Komatsuda, Guoxiong Chen |
Abstract |
The barley eceriferum-b.2 (cer-b.2) mutant produces glossy leaf sheaths and is deficient in the cuticular wax component 14,16-hentriacontanedione. The mutated gene maps to a 1.3-cM interval on chromosome 3HL flanked by the genes MLOC_10972 and MLOC_69561. The cuticular wax coating of leaves and stems in many grass species is responsible for the plants' glaucous appearance. A major component of the wax is a group of β-diketone compounds. The barley eceriferum-b.2 (cer-b.2) mutant produces glossy leaf sheaths and is deficient for the compound 14,16-hentriacontanedione. A linkage analysis based on 708 gametes allowed the gene responsible for the mutant phenotype to be mapped to a 1.3-cM interval on chromosome 3HL flanked by the two genes MLOC_10972 and _69561. The product of the wild type allele may represent a step in the β-diketone synthesis pathway. |
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