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The use of the rapid osmotic fragility test as an additional test to diagnose canine immune-mediated haemolytic anaemia

Overview of attention for article published in Acta Veterinaria Scandinavica, October 2013
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Title
The use of the rapid osmotic fragility test as an additional test to diagnose canine immune-mediated haemolytic anaemia
Published in
Acta Veterinaria Scandinavica, October 2013
DOI 10.1186/1751-0147-55-74
Pubmed ID
Authors

Geert Paes, Dominique Paepe, Evelyne Meyer, Annemarie T Kristensen, Luc Duchateau, Miguel Campos, Sylvie Daminet

Abstract

Diagnosing canine immune-mediated haemolytic anaemia (IMHA) is often challenging because all currently available tests have their limitations. Dogs with IMHA often have an increased erythrocyte osmotic fragility (OF), a characteristic that is sometimes used in the diagnosis of IMHA. Since the classic osmotic fragility test (COFT) is time-consuming and requires specialized equipment, an easy and less labour-intensive rapid osmotic fragility test (ROFT) has been used in some countries, but its diagnostic value has not yet been investigated.This study aimed to evaluate erythrocyte osmotic fragility in dogs with and without IMHA, to compare results of the classic (COFT) and rapid (ROFT) test and to assess the value of the ROFT as diagnostic test for canine IMHA.Nineteen dogs with IMHA (group 1a), 21 anaemic dogs without IMHA (group 1b), 8 dogs with microcytosis (group 2), 13 hyperlipemic dogs (group 3), 10 dogs with lymphoma (group 4), 8 dogs with an infection (group 5) and 13 healthy dogs (group 6) were included.In all dogs, blood smear examination, in-saline auto-agglutination test, Coombs' test, COFT and ROFT were performed. In the COFT, OF5, OF50 and OF90 were defined as the NaCl concentrations at which respectively 5, 50 and 90% of erythrocytes were haemolysed.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Italy 1 1%
Unknown 71 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 14 19%
Other 11 15%
Student > Master 8 11%
Professor 4 6%
Student > Postgraduate 4 6%
Other 10 14%
Unknown 21 29%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 25 35%
Medicine and Dentistry 14 19%
Immunology and Microbiology 3 4%
Nursing and Health Professions 3 4%
Unspecified 1 1%
Other 2 3%
Unknown 24 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 November 2013.
All research outputs
#19,945,185
of 25,374,917 outputs
Outputs from Acta Veterinaria Scandinavica
#506
of 837 outputs
Outputs of similar age
#161,941
of 224,583 outputs
Outputs of similar age from Acta Veterinaria Scandinavica
#6
of 16 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 837 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 224,583 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.