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Dictyostelium discoideum Protocols

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Cover of 'Dictyostelium discoideum Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 The Amoebozoa
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    Chapter 2 The Model Organism Dictyostelium discoideum.
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    Chapter 3 Comparative genomics of the dictyostelids.
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    Chapter 4 Dictyostelium discoideum Protocols
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    Chapter 5 Fluorescent reporters and methods to analyze fluorescent signals.
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    Chapter 6 Collection and cultivation of dictyostelids from the wild.
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    Chapter 7 Identification and Verification of microRNAs by High-Throughput Sequencing.
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    Chapter 8 Transcriptional profiling of dictyostelium with RNA sequencing.
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    Chapter 9 Analysis of chromatin organization by deep sequencing technologies.
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    Chapter 10 Pharmacogenetics of resistance to Cisplatin and other anticancer drugs and the role of sphingolipid metabolism.
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    Chapter 11 N-glycomic and N-glycoproteomic studies in the social amoebae.
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    Chapter 12 Measuring Cheating, Fitness, and Segregation in Dictyostelium discoideum.
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    Chapter 13 The Application of the Cre-loxP System for Generating Multiple Knock-out and Knock-in Targeted Loci.
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    Chapter 14 Extrachromosomal Inducible Expression
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    Chapter 15 Isolation of dictyostelium nuclei for light and electron microscopy.
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    Chapter 16 Investigation of DNA repair pathway activity.
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    Chapter 17 Transcript Localization in Dictyostelium discoideum Cells by RNA FISH.
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    Chapter 18 Analysis of mitochondrial gene expression.
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    Chapter 19 Mitochondrial respiratory complex function and the phenotypic consequences of dysfunction.
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    Chapter 20 Micropipette aspiration for studying cellular mechanosensory responses and mechanics.
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    Chapter 21 Quantitative analysis of phagocytosis and phagosome maturation.
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    Chapter 22 Dictyostelium discoideum Protocols
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    Chapter 23 Isolation of pathogen-containing vacuoles.
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    Chapter 24 Immunomagnetic purification of fluorescent legionella-containing vacuoles.
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    Chapter 25 Secretory lysosomes in dictyostelium: visualization, characterization, and dynamics.
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    Chapter 26 Monitoring autophagy in dictyostelium.
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