Title |
Spinal poly-GA inclusions in a C9orf72 mouse model trigger motor deficits and inflammation without neuron loss
|
---|---|
Published in |
Acta Neuropathologica, April 2017
|
DOI | 10.1007/s00401-017-1711-0 |
Pubmed ID | |
Authors |
Martin H. Schludi, Lore Becker, Lillian Garrett, Tania F. Gendron, Qihui Zhou, Franziska Schreiber, Bastian Popper, Leda Dimou, Tim M. Strom, Juliane Winkelmann, Anne von Thaden, Kristin Rentzsch, Stephanie May, Meike Michaelsen, Benjamin M. Schwenk, Jing Tan, Benedikt Schoser, Marianne Dieterich, Leonard Petrucelli, Sabine M. Hölter, Wolfgang Wurst, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe de Angelis, Thomas Klopstock, Thomas Arzberger, Dieter Edbauer |
Abstract |
Translation of the expanded (ggggcc)n repeat in C9orf72 patients with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) causes abundant poly-GA inclusions. To elucidate their role in pathogenesis, we generated transgenic mice expressing codon-modified (GA)149 conjugated with cyan fluorescent protein (CFP). Transgenic mice progressively developed poly-GA inclusions predominantly in motoneurons and interneurons of the spinal cord and brain stem and in deep cerebellar nuclei. Poly-GA co-aggregated with p62, Rad23b and the newly identified Mlf2, in both mouse and patient samples. Consistent with the expression pattern, 4-month-old transgenic mice showed abnormal gait and progressive balance impairment, but showed normal hippocampus-dependent learning and memory. Apart from microglia activation we detected phosphorylated TDP-43 but no neuronal loss. Thus, poly-GA triggers behavioral deficits through inflammation and protein sequestration that likely contribute to the prodromal symptoms and disease progression of C9orf72 patients. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 25% |
Unknown | 6 | 75% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 8 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 175 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 39 | 22% |
Student > Bachelor | 30 | 17% |
Student > Master | 17 | 10% |
Researcher | 16 | 9% |
Student > Doctoral Student | 11 | 6% |
Other | 18 | 10% |
Unknown | 44 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 50 | 29% |
Biochemistry, Genetics and Molecular Biology | 25 | 14% |
Agricultural and Biological Sciences | 19 | 11% |
Medicine and Dentistry | 11 | 6% |
Psychology | 5 | 3% |
Other | 16 | 9% |
Unknown | 49 | 28% |