Title |
Hyperglucagonaemia analysed by glucagon sandwich ELISA: nonspecific interference or truly elevated levels?
|
---|---|
Published in |
Diabetologia, June 2014
|
DOI | 10.1007/s00125-014-3283-z |
Pubmed ID | |
Authors |
Nicolai J. Wewer Albrechtsen, Bolette Hartmann, Simon Veedfald, Johanne A. Windeløv, Astrid Plamboeck, Kirstine N. Bojsen-Møller, Thomas Idorn, Bo Feldt-Rasmussen, Filip K. Knop, Tina Vilsbøll, Sten Madsbad, Carolyn F. Deacon, Jens J. Holst |
Abstract |
Hyperglucagonaemia is a characteristic of several clinical conditions (e.g. end-stage renal disease (ESRD), type 2 diabetes, obesity before and after Roux-en-Y gastric bypass (RYGB) and vagotomy with pyloroplasty), but the molecular nature of 'immunoreactive' glucagon is poorly characterised. The specific determination of fully processed, intact glucagon requires a 'sandwich' assay employing a combination of antibodies directed against both N- and C-termini. We compared a novel assay for intact glucagon with a highly sensitive C-terminal RIA (hitherto considered specific) to determine the extent to which the hyperglucagonaemia measured in clinical samples was caused by authentic glucagon. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Sweden | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Denmark | 2 | 2% |
Sweden | 1 | <1% |
Unknown | 118 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 31 | 26% |
Student > Ph. D. Student | 28 | 23% |
Student > Master | 15 | 12% |
Other | 10 | 8% |
Student > Bachelor | 7 | 6% |
Other | 13 | 11% |
Unknown | 17 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 53 | 44% |
Biochemistry, Genetics and Molecular Biology | 15 | 12% |
Agricultural and Biological Sciences | 14 | 12% |
Chemistry | 4 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 3% |
Other | 11 | 9% |
Unknown | 20 | 17% |