Chapter 1 Cloning Full-Length Transcripts and Transcript Variants Using 5′ and 3′ RACE

Chapter 2 Monitoring Gene Expression: Quantitative Real-Time RT-PCR

Chapter 3 Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases

Chapter 4 Northern Analysis of Gene Expression

Chapter 5 Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

Chapter 6 Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research

Chapter 7 Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research

Chapter 8 Strategies for Designing Transgenic DNA Constructs

Chapter 9 Purification of Plasmid and BAC Transgenic DNA Constructs

Chapter 10 Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production

Chapter 11 Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes

Chapter 12 Generation of General and Tissue-Specific Gene Knockout Mouse Models

Chapter 13 Adeno-associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism

Chapter 14 Modulation of lipoprotein metabolism by antisense technology: preclinical drug discovery methodology.

Chapter 15 Chromatin Immunoprecipitation

Chapter 16 Measurement of Lecithin–Cholesterol Acyltransferase Activity with the Use of a Peptide-Proteoliposome Substrate

Chapter 17 Native–Native 2D Gel Electrophoresis for HDL Subpopulation Analysis

Chapter 18 Western Blots