Title |
September through October 2010 multi-centre study in the Netherlands examining laboratory ability to detect enterovirus 68, an emerging respiratory pathogen
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Published in |
Journal of Virological Methods, March 2013
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DOI | 10.1016/j.jviromet.2013.02.010 |
Pubmed ID | |
Authors |
Giovanna Jaramillo-Gutierrez, Kimberley S.M. Benschop, Eric C.J. Claas, Arjan S. de Jong, Anton M. van Loon, Suzan D. Pas, Oscar Pontesilli, John W. Rossen, Caroline M.A. Swanink, Steven Thijsen, Adri G.M. van der Zanden, Harrie G.A.M. van der Avoort, Marion P.G. Koopmans, Adam Meijer |
Abstract |
During September and October 2010, the Dutch Public Health Institute detected an enterovirus (EV) 68 (EV68) epidemic in the Netherlands through general practitioner-based surveillance of acute respiratory infections. EV68 shares phenotypic and genotypic properties with human rhinovirus (HRV). Despite increased EV and HRV detections, Dutch clinical laboratories did not identify EV68. To assess the capability of Dutch clinical laboratories to detect EV68, ten laboratories with more than eight detected EV and HRV cases in September and October 2010 provided information about their detection algorithms and testing results for a 2010 Dutch EV68 strain. For EV detection mostly stool specimens (median 49%), respiratory specimens (median 27%) and cerebrospinal fluid (median 22%) were used. For HRV detection only respiratory specimens were used. Except for the Seeplex® RV15ACE EV-specific assay, all EV and 73% of HRV assays, including those of the Public Health Institute, were able to detect EV68. Two-step EV RT-PCR protocols were the most sensitive. Thus, laboratories might have misidentified EV68 as HRV. In addition, EV68 cases might have also been missed because patients with respiratory diseases are usually not tested for EV infection. Therefore, clinical laboratories should include EV detection in the differential diagnosis of patients presenting with respiratory symptoms. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Netherlands | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 25% |
Researcher | 5 | 18% |
Student > Bachelor | 3 | 11% |
Other | 2 | 7% |
Student > Doctoral Student | 1 | 4% |
Other | 4 | 14% |
Unknown | 6 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 6 | 21% |
Agricultural and Biological Sciences | 6 | 21% |
Immunology and Microbiology | 4 | 14% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Unspecified | 1 | 4% |
Other | 3 | 11% |
Unknown | 6 | 21% |