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Genotyping-by-sequencing based intra-specific genetic map refines a ‘‘QTL-hotspot” region for drought tolerance in chickpea

Overview of attention for article published in Molecular Genetics and Genomics, October 2014
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Title
Genotyping-by-sequencing based intra-specific genetic map refines a ‘‘QTL-hotspot” region for drought tolerance in chickpea
Published in
Molecular Genetics and Genomics, October 2014
DOI 10.1007/s00438-014-0932-3
Pubmed ID
Authors

Deepa Jaganathan, Mahendar Thudi, Sandip Kale, Sarwar Azam, Manish Roorkiwal, Pooran M. Gaur, P B Kavi Kishor, Henry Nguyen, Tim Sutton, Rajeev K. Varshney

Abstract

To enhance the marker density in the "QTL-hotspot" region, harboring several QTLs for drought tolerance-related traits identified on linkage group 04 (CaLG04) in chickpea recombinant inbred line (RIL) mapping population ICC 4958 × ICC 1882, a genotyping-by-sequencing approach was adopted. In total, 6.24 Gb data from ICC 4958, 5.65 Gb data from ICC 1882 and 59.03 Gb data from RILs were generated, which identified 828 novel single-nucleotide polymorphisms (SNPs) for genetic mapping. Together with these new markers, a high-density intra-specific genetic map was developed that comprised 1,007 marker loci spanning a distance of 727.29 cM. QTL analysis using the extended genetic map along with precise phenotyping data for 20 traits collected over one to seven seasons identified 49 SNP markers in the "QTL-hotspot" region. These efforts have refined the "QTL-hotspot" region to 14 cM. In total, 164 main-effect QTLs including 24 novel QTLs were identified. In addition, 49 SNPs integrated in the "QTL-hotspot" region were converted into cleaved amplified polymorphic sequence (CAPS) and derived CAPS (dCAPS) markers which can be used in marker-assisted breeding.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 133 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 3 2%
Italy 1 <1%
Argentina 1 <1%
Unknown 128 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 28 21%
Researcher 28 21%
Student > Doctoral Student 14 11%
Student > Master 13 10%
Student > Bachelor 7 5%
Other 12 9%
Unknown 31 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 79 59%
Biochemistry, Genetics and Molecular Biology 14 11%
Environmental Science 2 2%
Medicine and Dentistry 2 2%
Unspecified 1 <1%
Other 5 4%
Unknown 30 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 October 2014.
All research outputs
#17,302,400
of 25,394,764 outputs
Outputs from Molecular Genetics and Genomics
#2,753
of 3,321 outputs
Outputs of similar age
#164,276
of 273,574 outputs
Outputs of similar age from Molecular Genetics and Genomics
#14
of 32 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,321 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 273,574 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.