↓ Skip to main content

Increased resistance to cucumber mosaic virus (CMV) in Lilium transformed with a defective CMV replicase gene

Overview of attention for article published in Biotechnology Techniques, February 2011
Altmetric Badge

Mentioned by

peer_reviews
1 peer review site

Citations

dimensions_citation
37 Dimensions

Readers on

mendeley
18 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Increased resistance to cucumber mosaic virus (CMV) in Lilium transformed with a defective CMV replicase gene
Published in
Biotechnology Techniques, February 2011
DOI 10.1007/s10529-011-0550-7
Pubmed ID
Authors

Pejman Azadi, Ntui Valentaine Otang, Hasthanasombut Supaporn, Raham Sher Khan, Dong Poh Chin, Ikuo Nakamura, Masahiro Mii

Abstract

Lilium cv Acapulco was transformed with a defective cucumber mosaic virus (CMV) replicase gene (CMV2-GDD) construct using Agrobacterium tumefaciens. Four lines were analyzed for gene expression and resistance to CMV-O strain. Expression of the CMV2-GDD gene in the transgenic plants was confirmed by reverse transcription PCR (RT-PCR). When these four lines were mechanically inoculated with CMV-O, no signal of coat protein (CP) messages using RT-PCR was detected in newly produced leaves of two transgenic lines. Dot-immunobinding assay (DIBA) of CP was performed to examine the presence of the CMV in the newly produced leaves of challenged plants. Results, similar to those obtained with RT-PCR of the CP messages, were observed in DIBA. Therefore, our results imply that the two lines show increased levels of resistance to CMV, and CMV-GDD replicase gene is an effective construct that has protection against CMV in Lilium.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 6%
Unknown 17 94%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 39%
Student > Bachelor 3 17%
Researcher 3 17%
Student > Ph. D. Student 2 11%
Unknown 3 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 44%
Biochemistry, Genetics and Molecular Biology 5 28%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Economics, Econometrics and Finance 1 6%
Unknown 3 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 July 2015.
All research outputs
#17,286,379
of 25,374,647 outputs
Outputs from Biotechnology Techniques
#2,205
of 2,762 outputs
Outputs of similar age
#153,823
of 193,376 outputs
Outputs of similar age from Biotechnology Techniques
#29
of 32 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 193,376 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.