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Synthetic Metabolic Pathways

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Cover of 'Synthetic Metabolic Pathways'

Table of Contents

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    Book Overview
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    Chapter 1 Parts Characterization for Tunable Protein Expression
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    Chapter 2 Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design
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    Chapter 3 Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability
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    Chapter 4 An Automated Pipeline for Engineering Many-Enzyme Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization
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    Chapter 5 Computational Approaches on Stoichiometric and Kinetic Modeling for Efficient Strain Design
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    Chapter 6 Extended Metabolic Space Modeling
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    Chapter 7 Computational Methods to Assess the Production Potential of Bio-Based Chemicals
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    Chapter 8 Multiplex Genome Editing in Escherichia coli
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    Chapter 9 Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-Gene Systems
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    Chapter 10 An Adaptive Laboratory Evolution Method to Accelerate Autotrophic Metabolism
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    Chapter 11 CRISPR-Cas9 Toolkit for Actinomycete Genome Editing
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    Chapter 12 Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast Using CasEMBLR
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    Chapter 13 A Modified Gibson Assembly Method for Cloning Large DNA Fragments with High GC Contents
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    Chapter 14 Coupling Yeast Golden Gate and VEGAS for Efficient Assembly of the Violacein Pathway in Saccharomyces cerevisiae
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    Chapter 15 Multi-capillary Column Ion Mobility Spectrometry of Volatile Metabolites for Phenotyping of Microorganisms
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    Chapter 16 Selection of Highly Expressed Gene Variants in Escherichia coli Using Translationally Coupled Antibiotic Selection Markers
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    Chapter 17 Design, Engineering, and Characterization of Prokaryotic Ligand-Binding Transcriptional Activators as Biosensors in Yeast
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    Chapter 18 A Capture-SELEX Strategy for Multiplexed Selection of RNA Aptamers Against Small Molecules
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    Chapter 19 High-Throughput Microfluidics for the Screening of Yeast Libraries
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    Chapter 20 Growth-Coupled Carotenoids Production Using Adaptive Laboratory Evolution
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    Chapter 21 Two-Scale 13C Metabolic Flux Analysis for Metabolic Engineering
Attention for Chapter 4: An Automated Pipeline for Engineering Many-Enzyme Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization
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