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Engineering genome-reduced Bacillus subtilis for acetoin production from xylose

Overview of attention for article published in Biotechnology Techniques, December 2017
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Title
Engineering genome-reduced Bacillus subtilis for acetoin production from xylose
Published in
Biotechnology Techniques, December 2017
DOI 10.1007/s10529-017-2481-4
Pubmed ID
Authors

Panpan Yan, Yuanqing Wu, Li Yang, Zhiwen Wang, Tao Chen

Abstract

To investigate the capacity of a genome-reduced Bacillus subtilis strain as chassis cell for acetoin production from xylose. To endow the genome-reduced Bacillus subtilis strain BSK814 with the ability to utilize xylose, we inserted a native xyl operon into its genome and deleted the araR gene. The resulting strain BSK814A2 produced 2.94 g acetoin/l from 10 g xylose/l, which was 39% higher than control strain BSK19A2. The deletion of the bdhA and acoA genes further improved xylose utilization efficiency and increased acetoin production to 3.71 g/l in BSK814A4. Finally, BSK814A4 produced up to 23.3 g acetoin/l from 50 g xylose/l, with a yield of 0.46 g/g xylose. Both the titer and yield were 39% higher than those of control strain BSK19A4. As a chassis cell, genome-reduced B. subtilis showed significantly improved capacity for the production of the overflow product acetoin from xylose compared with wild-type strain.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 24%
Student > Master 5 24%
Student > Doctoral Student 3 14%
Researcher 2 10%
Student > Bachelor 1 5%
Other 1 5%
Unknown 4 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 43%
Agricultural and Biological Sciences 3 14%
Immunology and Microbiology 1 5%
Psychology 1 5%
Physics and Astronomy 1 5%
Other 1 5%
Unknown 5 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2018.
All research outputs
#22,764,772
of 25,382,440 outputs
Outputs from Biotechnology Techniques
#2,493
of 2,762 outputs
Outputs of similar age
#383,469
of 443,420 outputs
Outputs of similar age from Biotechnology Techniques
#17
of 21 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.