Title |
Acute or Delayed Systemic Administration of Human Amnion Epithelial Cells Improves Outcomes in Experimental Stroke
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Published in |
Stroke, January 2018
|
DOI | 10.1161/strokeaha.117.019136 |
Pubmed ID | |
Authors |
Megan A Evans, Rebecca Lim, Hyun Ah Kim, Hannah X Chu, Chantelle V Gardiner-Mann, Kimberly W E Taylor, Christopher T Chan, Vanessa H Brait, Seyoung Lee, Quynh Nhu Dinh, Antony Vinh, Thanh G Phan, Velandai K Srikanth, Henry Ma, Thiruma V Arumugam, David Y Fann, Luting Poh, Cameron P J Hunt, Colin W Pouton, John M Haynes, Stavros Selemidis, William Kwan, Leon Teo, James A Bourne, Silke Neumann, Sarah Young, Emma K Gowing, Grant R Drummond, Andrew N Clarkson, Euan M Wallace, Christopher G Sobey, Brad R S Broughton |
Abstract |
Human amnion epithelial cells (hAECs) are nonimmunogenic, nontumorigenic, anti-inflammatory cells normally discarded with placental tissue. We reasoned that their profile of biological features, wide availability, and the lack of ethical barriers to their use could make these cells useful as a therapy in ischemic stroke. We tested the efficacy of acute (1.5 hours) or delayed (1-3 days) poststroke intravenous injection of hAECs in 4 established animal models of cerebral ischemia. Animals included young (7-14 weeks) and aged mice (20-22 months) of both sexes, as well as adult marmosets of either sex. We found that hAECs administered 1.5 hours after stroke in mice migrated to the ischemic brain via a CXC chemokine receptor type 4-dependent mechanism and reduced brain inflammation, infarct development, and functional deficits. Furthermore, if hAECs administration was delayed until 1 or 3 days poststroke, long-term functional recovery was still augmented in young and aged mice of both sexes. We also showed proof-of-principle evidence in marmosets that acute intravenous injection of hAECs prevented infarct development from day 1 to day 10 after stroke. Systemic poststroke administration of hAECs elicits marked neuroprotection and facilitates mechanisms of repair and recovery. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 14% |
Korea, Republic of | 1 | 14% |
Australia | 1 | 14% |
Nigeria | 1 | 14% |
United States | 1 | 14% |
Unknown | 2 | 29% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 5 | 71% |
Practitioners (doctors, other healthcare professionals) | 2 | 29% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 58 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 12% |
Researcher | 7 | 12% |
Student > Master | 7 | 12% |
Student > Bachelor | 6 | 10% |
Professor | 3 | 5% |
Other | 8 | 14% |
Unknown | 20 | 34% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 10 | 17% |
Neuroscience | 6 | 10% |
Engineering | 6 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 7% |
Biochemistry, Genetics and Molecular Biology | 4 | 7% |
Other | 5 | 9% |
Unknown | 23 | 40% |