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An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro

Overview of attention for article published in Malaria Journal, February 2018
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Title
An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro
Published in
Malaria Journal, February 2018
DOI 10.1186/s12936-018-2208-6
Pubmed ID
Authors

Takahiro Tougan, Yuhgi Suzuki, Sawako Itagaki, Munehisa Izuka, Yuji Toya, Kinya Uchihashi, Toshihiro Horii

Abstract

The automated haematology analyzer XN-30 (Sysmex, Kobe, Japan) easily and rapidly detects malarial parasites in clinical blood samples using flow cytometry. The XN-30 analyzer is able to distinguish each developmental stage by measuring DNA content and cell size. Thus, it was expected to be capable of quantifying the developmental stages of cultured falciparum parasite. To achieve this requirement, a modified algorithm was tested for its validity and reliability using in vitro cultured falciparum parasite. The XN-30 analyzer automatically measured each developmental stage as well as total parasitaemia. Comparison of the parasitaemia obtained using the XN-30 analyzer equipped with the modified algorithm with that obtained using microscopy examination of Giemsa-stained smears revealed the greater sensitivity and reproducibility of the former. The XN-30 analyzer also detected free merozoites and purified gametocytes. The XN-30 analyzer allows the precise recognition and enumeration of total and each developmental stages of cultured falciparum parasites, and permits the sensitive and reproducible calculation of parasitaemia. The results indicate the potential of the XN-30 analyzer for basic research on malarial biology, anti-malarial drug discovery, and evaluation of drug efficacy.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 38 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 24%
Student > Master 4 11%
Researcher 4 11%
Student > Bachelor 4 11%
Student > Doctoral Student 3 8%
Other 4 11%
Unknown 10 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 16%
Medicine and Dentistry 5 13%
Immunology and Microbiology 5 13%
Biochemistry, Genetics and Molecular Biology 3 8%
Chemistry 3 8%
Other 6 16%
Unknown 10 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 January 2019.
All research outputs
#15,490,822
of 23,020,670 outputs
Outputs from Malaria Journal
#4,505
of 5,598 outputs
Outputs of similar age
#269,121
of 439,370 outputs
Outputs of similar age from Malaria Journal
#106
of 125 outputs
Altmetric has tracked 23,020,670 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,598 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 439,370 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 125 others from the same source and published within six weeks on either side of this one. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.