Title |
Regeneration of functional alveoli by adult human SOX9+ airway basal cell transplantation
|
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Published in |
Protein & Cell, January 2018
|
DOI | 10.1007/s13238-018-0506-y |
Pubmed ID | |
Authors |
Qiwang Ma, Yu Ma, Xiaotian Dai, Tao Ren, Yingjie Fu, Wenbin Liu, Yufei Han, Yingchuan Wu, Yu Cheng, Ting Zhang, Wei Zuo |
Abstract |
Irreversible destruction of bronchi and alveoli can lead to multiple incurable lung diseases. Identifying lung stem/progenitor cells with regenerative capacity and utilizing them to reconstruct functional tissue is one of the biggest hopes to reverse the damage and cure such diseases. Here we showed that a rare population of SOX9+ basal cells (BCs) located at airway epithelium rugae can regenerate adult human lung. Human SOX9+ BCs can be readily isolated by bronchoscopic brushing and indefinitely expanded in feeder-free condition. Expanded human SOX9+ BCs can give rise to alveolar and bronchiolar epithelium after being transplanted into injured mouse lung, with air-blood exchange system reconstructed and recipient's lung function improved. Manipulation of lung microenvironment with Pirfenidone to suppress TGF-β signaling could further boost the transplantation efficiency. Moreover, we conducted the first autologous SOX9+ BCs transplantation clinical trial in two bronchiectasis patients. Lung tissue repair and pulmonary function enhancement was observed in patients 3-12 months after cell transplantation. Altogether our current work indicated that functional adult human lung structure can be reconstituted by orthotopic transplantation of tissue-specific stem/progenitor cells, which could be translated into a mature regenerative therapeutic strategy in near future. |
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Geographical breakdown
Country | Count | As % |
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United States | 5 | 24% |
France | 4 | 19% |
Mexico | 1 | 5% |
Guinea | 1 | 5% |
Spain | 1 | 5% |
Moldova, Republic of | 1 | 5% |
Germany | 1 | 5% |
Unknown | 7 | 33% |
Demographic breakdown
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Members of the public | 16 | 76% |
Scientists | 5 | 24% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 95 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 21 | 22% |
Researcher | 12 | 13% |
Student > Bachelor | 9 | 9% |
Student > Master | 8 | 8% |
Student > Postgraduate | 4 | 4% |
Other | 15 | 16% |
Unknown | 26 | 27% |
Readers by discipline | Count | As % |
---|---|---|
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Agricultural and Biological Sciences | 14 | 15% |
Biochemistry, Genetics and Molecular Biology | 13 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 6 | 6% |
Immunology and Microbiology | 4 | 4% |
Other | 12 | 13% |
Unknown | 28 | 29% |