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Site-Specific Protein Labeling

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Cover of 'Site-Specific Protein Labeling'

Table of Contents

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    Book Overview
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    Chapter 1 How FlAsH Got Its Sparkle: Historical Recollections of the Biarsenical-Tetracysteine Tag
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    Chapter 2 Site-specific protein labeling in the pharmaceutical industry: experiences from novartis drug discovery.
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    Chapter 3 Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins
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    Chapter 4 Considerations and protocols for the synthesis of custom protein labeling probes.
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    Chapter 5 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues.
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    Chapter 6 Fluorescent Labeling for Patch-Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels
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    Chapter 7 Fluorescent Labeling of SNAP-Tagged Proteins in Cells.
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    Chapter 8 HaloTag Technology for Specific and Covalent Labeling of Fusion Proteins
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    Chapter 9 Ligation of Synthetic Peptides to Proteins Using Semisynthetic Protein trans -Splicing
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    Chapter 10 Chemical-Tag Labeling of Proteins Using Fully Recombinant Split Inteins
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    Chapter 11 Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase-Catalyzed Site-Specific Protein Labeling
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    Chapter 12 Site-Specific Biotinylation of Purified Proteins Using BirA
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    Chapter 13 Site-Specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist
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    Chapter 14 BONCAT: Metabolic Labeling, Click Chemistry, and Affinity Purification of Newly Synthesized Proteomes.
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    Chapter 15 Genetic Encoding of Unnatural Amino Acids for Labeling Proteins
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    Chapter 16 Labeling Proteins by Affinity-Guided DMAP Chemistry
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    Chapter 17 Ligand-Directed Tosyl Chemistry for Selective Native Protein Labeling In Vitro, In Cells, and In Vivo
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