Angiogenesis Protocols

Chapter 1 Angiogenic Signalling Pathways

Chapter 2 Angiogenic Signalling Pathways

Chapter 3 Assessing Tumor Angiogenesis in Histological Samples

Chapter 4 Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis

Chapter 5 Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Culture

Chapter 6 Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines

Chapter 7 Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells

Chapter 8 Circulating Endothelial Progenitor Cells (CEPC)

Chapter 9 In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and Proteolysis

Chapter 10 Tube Formation: an In Vitro Matrigel Angiogenesis Assay

Chapter 11 Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model

Chapter 12 Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature

Chapter 13 Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction

Chapter 14 Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound Healing

Chapter 15 Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay

Chapter 16 Quantitative estimation of tissue blood flow rate.

Chapter 17 In Vivo Matrigel Migration and Angiogenesis Assay

Chapter 18 The Sponge Implant Model of Angiogenesis

Chapter 19 The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital Microscopy

Chapter 20 The Corneal Pocket Assay

Chapter 21 Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature

Chapter 22 The Cranial Bone Window Model: Studying Angiogenesis of Primary and Secondary Bone Tumors by Intravital Microscopy