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Mendeley readers
Attention Score in Context
| Title |
Cloning, expression, and PCR application of DNA polymerase from the hyperthermophilic archaeon, Thermococcus celer
|
|---|---|
| Published in |
Biotechnology Techniques, October 2010
|
| DOI | 10.1007/s10529-010-0434-2 |
| Pubmed ID | |
| Authors |
Kee Pum Kim, Heejin Bae, In Hye Kim, Suk-Tae Kwon |
| Abstract |
The family B DNA polymerase gene was amplified from Thermococcus celer genomic DNA by using the degenerate primers and DNA walking PCR. The Tce DNA polymerase gene was cloned and sequenced. The gene contains an ORF of 2,325 bp encoding 774 amino acid residues with a calculated molecular weight of 89,788.9 kDa. The Tce DNA polymerase was purified by heat treatment and heparin column chromatography. The optimal conditions for PCR were determined. Long-range PCR and time-saving PCR were performed using various specific ratios of Taq and Tce DNA polymerases (Tce plus DNA polymerase). Tce plus DNA polymerase surpassed the PCR performance of Tce, Taq and Pfu DNA polymerases in terms of yield and efficiency. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 28% |
| Researcher | 4 | 22% |
| Professor | 2 | 11% |
| Student > Bachelor | 2 | 11% |
| Student > Postgraduate | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 4 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 50% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Nursing and Health Professions | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Unknown | 5 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2016.
All research outputs
#8,533,995
of 25,371,288 outputs
Outputs from Biotechnology Techniques
#827
of 2,762 outputs
Outputs of similar age
#39,525
of 108,403 outputs
Outputs of similar age from Biotechnology Techniques
#18
of 32 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 108,403 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.