Title |
Detection of bat coronaviruses from Miniopterus fuliginosus in Japan
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Published in |
Virus Genes, August 2011
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DOI | 10.1007/s11262-011-0661-1 |
Pubmed ID | |
Authors |
Kazuya Shirato, Ken Maeda, Shumpei Tsuda, Kazuo Suzuki, Shumpei Watanabe, Hiroshi Shimoda, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Shigeru Kyuwa, Daiji Endoh, Shutoku Matsuyama, Ichiro Kurane, Masayuki Saijo, Shigeru Morikawa, Yasuhiro Yoshikawa, Hiroomi Akashi, Tetsuya Mizutani |
Abstract |
Bats have great potential as reservoirs for emerging viruses such as severe acute respiratory syndrome-coronavirus. In this study, bat coronaviruses (BtCoVs) were detected by RT-PCR from intestinal and fecal specimens of Miniopterus fuliginosus breeding colonies in Wakayama Prefecture caves, where we previously identified bat betaherpesvirus 2. Two primer sets were used for the detection of BtCoV: one was for the RNA-dependent RNA polymerase (RdRp) region and the other was for the spike (S) protein region. Eleven and 73% of intestinal and fecal specimens, respectively, were positive for RdRp region, and 2 and 40% of those were positive for S protein region. Sequencing and phylogenetic analysis showed that the detected BtCoV belonged to the group 1 (alpha) coronaviruses. These data suggest that BtCoV is endemic in M. fuliginosus in Japan. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 2 | 3% |
Japan | 1 | 2% |
United States | 1 | 2% |
China | 1 | 2% |
Unknown | 56 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 17 | 28% |
Researcher | 10 | 16% |
Student > Master | 9 | 15% |
Professor | 6 | 10% |
Student > Bachelor | 5 | 8% |
Other | 8 | 13% |
Unknown | 6 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 32 | 52% |
Biochemistry, Genetics and Molecular Biology | 4 | 7% |
Medicine and Dentistry | 4 | 7% |
Environmental Science | 3 | 5% |
Immunology and Microbiology | 3 | 5% |
Other | 5 | 8% |
Unknown | 10 | 16% |