| Title |
Comprehensive serological profiling of human populations using a synthetic human virome
|
|---|---|
| Published in |
Science, June 2015
|
| DOI | 10.1126/science.aaa0698 |
| Pubmed ID | |
| Authors |
George J Xu, Tomasz Kula, Qikai Xu, Mamie Z Li, Suzanne D Vernon, Thumbi Ndung'u, Kiat Ruxrungtham, Jorge Sanchez, Christian Brander, Raymond T Chung, Kevin C O'Connor, Bruce Walker, H Benjamin Larman, Stephen J Elledge |
| Abstract |
The human virome plays important roles in health and immunity. However, current methods for detecting viral infections and antiviral responses have limited throughput and coverage. Here, we present VirScan, a high-throughput method to comprehensively analyze antiviral antibodies using immunoprecipitation and massively parallel DNA sequencing of a bacteriophage library displaying proteome-wide peptides from all human viruses. We assayed over 10(8) antibody-peptide interactions in 569 humans across four continents, nearly doubling the number of previously established viral epitopes. We detected antibodies to an average of 10 viral species per person and 84 species in at least two individuals. Although rates of specific virus exposure were heterogeneous across populations, antibody responses targeted strongly conserved "public epitopes" for each virus, suggesting that they may elicit highly similar antibodies. VirScan is a powerful approach for studying interactions between the virome and the immune system. |
X Demographics
The data shown below were collected from the profiles of 444 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 149 | 34% |
| United Kingdom | 28 | 6% |
| Canada | 15 | 3% |
| Spain | 13 | 3% |
| France | 10 | 2% |
| Australia | 8 | 2% |
| India | 6 | 1% |
| Mexico | 5 | 1% |
| Colombia | 3 | <1% |
| Other | 41 | 9% |
| Unknown | 166 | 37% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 291 | 66% |
| Scientists | 104 | 23% |
| Practitioners (doctors, other healthcare professionals) | 34 | 8% |
| Science communicators (journalists, bloggers, editors) | 15 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 971 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 18 | 2% |
| Germany | 3 | <1% |
| Netherlands | 3 | <1% |
| Japan | 3 | <1% |
| Switzerland | 2 | <1% |
| Israel | 2 | <1% |
| United Kingdom | 2 | <1% |
| Vietnam | 1 | <1% |
| Australia | 1 | <1% |
| Other | 10 | 1% |
| Unknown | 926 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 246 | 25% |
| Student > Ph. D. Student | 210 | 22% |
| Student > Master | 76 | 8% |
| Other | 55 | 6% |
| Student > Bachelor | 54 | 6% |
| Other | 179 | 18% |
| Unknown | 151 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 282 | 29% |
| Biochemistry, Genetics and Molecular Biology | 143 | 15% |
| Medicine and Dentistry | 118 | 12% |
| Immunology and Microbiology | 102 | 11% |
| Engineering | 30 | 3% |
| Other | 108 | 11% |
| Unknown | 188 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1059. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2023.
All research outputs
#15,019
of 25,791,495 outputs
Outputs from Science
#757
of 83,338 outputs
Outputs of similar age
#98
of 281,624 outputs
Outputs of similar age from Science
#8
of 1,344 outputs
Altmetric has tracked 25,791,495 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 83,338 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 66.0. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,624 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1,344 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.