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Fluorescence polarization immunoassay for rapid screening of the pesticides thiabendazole and tetraconazole in wheat

Overview of attention for article published in Analytical & Bioanalytical Chemistry, August 2018
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Title
Fluorescence polarization immunoassay for rapid screening of the pesticides thiabendazole and tetraconazole in wheat
Published in
Analytical & Bioanalytical Chemistry, August 2018
DOI 10.1007/s00216-018-1296-z
Pubmed ID
Authors

Anna Yu. Boroduleva, Juan J. Manclús, Ángel Montoya, Sergei A. Eremin

Abstract

Fluorescence polarization immunoassays (FPIAs) for thiabendazole and tetraconazole were first developed. Tracers for FPIAs of thiabendazole and tetraconazole were synthesized and the tracers' structures were confirmed by HPLC-MS/MS. The 4-aminomethylfluorescein-labeled tracers allowed achieving the best assay sensitivity and minimum reagent consumption in comparison with aminofluorescein-labeled and alkyldiaminefluoresceinthiocarbamyl-labeled tracers. Measurements of fluorescence polarization were performed using a portable device. The developed FPIA methods were applied for the analysis of wheat. Fast and simple sample preparation technique earlier developed by authors for pesticides was adapted for thiabendazole and tetraconazole. The limits of detection of thiabendazole and tetraconazole in wheat were 20 and 200 μg/kg, and the lower limits of quantification were 40 and 600 μg/kg, respectively. The recovery test was performed by two methods-FPIA and HPLC-MS/MS. The results obtained by FPIA correlated well with those obtained by HPLC-MS/MS (r2 = 0.9985 for thiabendazole, r2 = 0.9952 for tetraconazole). Average recoveries of thiabendazole and tetraconazole were 74 ± 4% and 72 ± 3% by FPIA, and average recoveries of thiabendazole and tetraconazole were 86 ± 2% and 74 ± 1% by HPLC-MS/MS (n = 15). Graphical abstract ᅟ.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 44%
Professor > Associate Professor 1 11%
Student > Postgraduate 1 11%
Student > Bachelor 1 11%
Unknown 2 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 22%
Biochemistry, Genetics and Molecular Biology 1 11%
Immunology and Microbiology 1 11%
Chemistry 1 11%
Engineering 1 11%
Other 0 0%
Unknown 3 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 April 2019.
All research outputs
#19,954,338
of 25,385,509 outputs
Outputs from Analytical & Bioanalytical Chemistry
#6,061
of 9,619 outputs
Outputs of similar age
#249,564
of 341,399 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#89
of 172 outputs
Altmetric has tracked 25,385,509 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,619 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 341,399 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 172 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.